Illuminating the dark side of the human transcriptome with TAMA Iso-Seq analysis
Illuminating the dark side of the human transcriptome with TAMA Iso-Seq analysis
Abstract The human transcriptome is one of the most well-annotated of the eukaryotic species. However, limitations in technology biased discovery toward protein coding spliced genes. Accurate high throughput long read RNA sequencing now has the potential to investigate genes that were previously undetectable. Using our Transcriptome Annotation by Modular Algorithms (TAMA) tool kit to analyze the Pacific Bioscience Universal Human Reference RNA Sequel II Iso-Seq dataset, we discovered thousands of potential novel genes and identified challenges in both RNA preparation and long read data processing that have major implications for transcriptome annotation.
Cheng Yuanyuan、Smith Jacqueline、Archibald Alan L.、Burt David W.、Kuo Richard I.
The University of Queensland||School of Life and Environmental Sciences, University of SydneyThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of EdinburghThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of EdinburghThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh||The University of QueenslandThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh
生物科学研究方法、生物科学研究技术遗传学分子生物学
Cheng Yuanyuan,Smith Jacqueline,Archibald Alan L.,Burt David W.,Kuo Richard I..Illuminating the dark side of the human transcriptome with TAMA Iso-Seq analysis[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/780015.点此复制
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