New insights in Ulcerative Colitis Associated Gut Microbiota in South American Population: Akkermansia and Collinsella , two distinctive genera found in Argentine subjects
New insights in Ulcerative Colitis Associated Gut Microbiota in South American Population: Akkermansia and Collinsella , two distinctive genera found in Argentine subjects
ABSTRACT BackgroundGlobally, ulcerative colitis (UC) is the most common form of intestinal inflammation, which is believed to be the result of a deregulated immune system response to commensal microbiota in a genetically susceptible host. Multicellular organisms rely heavily on their commensal symbiotic microbiota, whose composition is closely related to intrinsic local characteristics and regulated or modified by environmental factors. In the present study we aim to describe the unknown gut microbiota of patients with UC in comparison with healthy individuals in order to find novel biomarkers for UC in our region. MethodsWe evaluated 46 individuals, 26 healthy non-UC controls and 20 UC patients, from the metropolitan area of Buenos Aires (BA), Argentina. Clinical features, biochemical tests and anthropometric measurements were determined. Fecal samples were collected and DNA was extracted for microbiota analysis. The hypervariable regions V3-V4 of the bacterial 16SR gene were sequenced using a MiSeq platform and sequences were analyzed using the QIIME2 environment. In addition, we looked for differential functional pathways using PICRUSt and compared the performance of three machine learning models to discriminate the studied individuals, using taxa and functional annotations. ResultsAll UC patients were under clinical treatment with 70% of individuals in remission. We found no significant differences in gut microbiota richness or evenness between UC patients and non-UC controls (alpha diversity). Remarcably, microbial compositional structure within groups (beta diversity) showed differences: At the phylum level, Verrucomicrobia was overrepresented in controls while Actinobacteria was distinctive of UC patients; At the genus level Bacteroides and Akkermancia were significantly more abundant among controls while Eubacterium and Collinsella in UC patients. In addition, our results showed that carbohydrates metabolism was preponderant in UC patients, not observing a distinctive biochemical pathway for the healthy non-UC controls. Finally, in order to define a robust classifying method in our population, we evaluated the capability of three machine learning models to classify individuals. Our results reinforced the idea of functional compensation in microbiome communities, as models that used KEGG orthologs annotations had better capabilities than taxonomy to distinguish UC patients. ConclusionsOur study provides new knowledge on the differences and similarities of the gut microbiota of UC patients as compared to non-UC controls of our population. This allows not only the association of local changes in gut microbial diversity with the pathology process, but also the future development of personalized nutritional and pharmacological therapies through the use of omic strategies describing the metagenomic profiles of the Argentine population.
Ayel¨|n Rosso、Renata Spiazzi、Federico Coluccio Leskow、Iraola Gregorio、Jimena Cerezo、Alberto Penas-Steinhardt、Pablo Aguilera、Claudia Milano、Belforte Fiorella S.、Sof¨aa Quesada、Carolina Conlon
Laboratorio de Gen¨?mica Computacional (GeC-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠n, Luj¨￠n||Programa del Estudio de Comunicaci¨?n y Se?alizaci¨?n Interreino (PECSI-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠nServicio de Gastroenterolog¨aa, Hospital Nacional Prof. Alejandro PosadasPrograma del Estudio de Comunicaci¨?n y Se?alizaci¨?n Interreino (PECSI-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠n||Consejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas (CONICET)Laboratorio de Gen¨?mica Microbiana, Institut Pasteur Montevideo||Centro de Biolog¨aa Integrativa, Universidad Mayor||Wellcome Sanger Institute, Wellcome Genome CampusServicio de Gastroenterolog¨aa, Hospital Nacional Prof. Alejandro PosadasLaboratorio de Gen¨?mica Computacional (GeC-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠n, Luj¨￠n||Programa del Estudio de Comunicaci¨?n y Se?alizaci¨?n Interreino (PECSI-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠n||Consejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas (CONICET)||Instituto Universitario de Ciencias de la SaludPrograma del Estudio de Comunicaci¨?n y Se?alizaci¨?n Interreino (PECSI-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠n||Consejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas (CONICET)Servicio de Gastroenterolog¨aa, Hospital Nacional Prof. Alejandro PosadasLaboratorio de Gen¨?mica Computacional (GeC-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠n, Luj¨￠n||Programa del Estudio de Comunicaci¨?n y Se?alizaci¨?n Interreino (PECSI-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠n||Consejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas (CONICET)||Instituto de Ecolog¨aa y Desarrollo Sustentable (INEDES-CONICET-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠nLaboratorio de Gen¨?mica Computacional (GeC-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠n, Luj¨￠n||Programa del Estudio de Comunicaci¨?n y Se?alizaci¨?n Interreino (PECSI-UNLu), Departamento de Ciencias B¨￠sicas, Universidad Nacional de Luj¨￠n||Consejo Nacional de Investigaciones Cient¨aficas y T¨|cnicas (CONICET)Servicio de Gastroenterolog¨aa, Hospital Nacional Prof. Alejandro Posadas
医药卫生理论医学研究方法微生物学
Ayel¨|n Rosso,Renata Spiazzi,Federico Coluccio Leskow,Iraola Gregorio,Jimena Cerezo,Alberto Penas-Steinhardt,Pablo Aguilera,Claudia Milano,Belforte Fiorella S.,Sof¨aa Quesada,Carolina Conlon.New insights in Ulcerative Colitis Associated Gut Microbiota in South American Population: Akkermansia and Collinsella , two distinctive genera found in Argentine subjects[EB/OL].(2025-03-28)[2025-05-07].https://www.medrxiv.org/content/10.1101/2020.07.29.20164764.点此复制
评论