低振幅振动训练对帕金森小鼠黑质多巴胺能神经元的保护作用

Objective: To investigate the effects of low amplitude viration training on potential compensatory changes of tyrosine hydroxylase(TH) in the number of substantia nigra neurons and brain-derived neurotrophic factor (BDNF) in the striatum of MPTP-lesioned mouse, and to underly the possible molecular mechanisms for the LVT. Methods : Thirty-two male C57BL mice were equally randomized into normal saline (NS), PD model, PD plus low amplitude, low frequency viration training(LVT) and PD plus low amplitude, high frequency viration training (HVT). PD model was established with 1-methyl-4-phenyl-1,2 ,3 ,6-tetrahydropyridine (MPTP) in mice. Viration training(VT) was started 7 d after MPTP lesioning and continued for 4 W. After the training of VT, the changes in positive TH and the content of BDNF of animals were observed by immunohistochemical technique and ELASA respectively. Results : Compared with NS group , the numbers of TH immunoreactive cells and BDNF values in PD were significantly lower than those in the NS group (all P < 0. 01); the numbers in LVT group and HVT group were significantly higher compared with those in the PD. (all P < 0. 01); and the numbers were not significantly different between the two group and NS group( P >0. 05) . Correlation analysis showed a positive correlation between the count of TH and BDNF values ( r = 0. 970, P < 0. 01) . Conclusion : It is suggested that VT may lead to improvement in motor performance through adaptive changes of the basal ganglia circuitry, that is beneficial to the survival of dopaminergic neurons in the substantia nigra of PD mice, and the one of mechanism might be related to the up-regulation of BDNF expression in striatum.