Efficient Generation of Human Pluripotent Stem Cells from Frozen Cord Tissue via Chemical Reprogramming
Efficient Generation of Human Pluripotent Stem Cells from Frozen Cord Tissue via Chemical Reprogramming
ABSTRACT Chemical reprogramming presents an innovative approach for generating induced pluripotent stem cells (iPSCs), bypassing the genetic instability and safe concern associated with viral vector approach. We describe a novel, efficient chemical method for reprogramming human umbilical cord tissue-derived mesenchymal stem cells (MSCs) into induced pluripotent stem cells (iPSCs). Compared to previous sources like adipose tissue and skin, frozen umbilical cord tissue offers an abundant, non-invasive, long-term storable, and ethically sound cell source. Our findings not only showcase the feasibility and safety of utilizing chemical reprogramming on cells from frozen umbilical cords but also underscore its potential in regenerative medicine, especially for developing safer and more effective therapies for cardiovascular diseases.
Miller Bradley、Liu Chun、Han Lu、Mahnke Donna K.、Li Siqi、Minx Jake、Lincoln Joy、Nansubuga Coneria、Beyer Andreas、Multerer Abigail、Shen Mengcheng
Department of Physiology and Cancer Center, Medical College of Wisconsin||Department of Medicine and Cardiovascular Center, Medical College of WisconsinDepartment of Physiology and Cancer Center, Medical College of Wisconsin||Department of Medicine and Cardiovascular Center, Medical College of WisconsinDepartment of Pediatrics, Section of Pediatric Cardiology, Medical College of Wisconsin||The Herma Heart Institute, Children?ˉs WisconsinDepartment of Pediatrics, Section of Pediatric Cardiology, Medical College of Wisconsin||The Herma Heart Institute, Children?ˉs WisconsinDepartment of Pediatrics, Section of Pediatric Cardiology, Medical College of Wisconsin||The Herma Heart Institute, Children?ˉs WisconsinDepartment of Physiology and Cancer Center, Medical College of Wisconsin||Department of Medicine and Cardiovascular Center, Medical College of WisconsinDepartment of Pediatrics, Section of Pediatric Cardiology, Medical College of Wisconsin||The Herma Heart Institute, Children?ˉs WisconsinDepartment of Physiology and Cancer Center, Medical College of Wisconsin||Department of Medicine and Cardiovascular Center, Medical College of WisconsinDepartment of Medicine and Cardiovascular Center, Medical College of WisconsinDepartment of Pediatrics, Section of Pediatric Cardiology, Medical College of Wisconsin||The Herma Heart Institute, Children?ˉs WisconsinStanford Cardiovascular Institute, Stanford University
基础医学生物科学研究方法、生物科学研究技术生物工程学
Miller Bradley,Liu Chun,Han Lu,Mahnke Donna K.,Li Siqi,Minx Jake,Lincoln Joy,Nansubuga Coneria,Beyer Andreas,Multerer Abigail,Shen Mengcheng.Efficient Generation of Human Pluripotent Stem Cells from Frozen Cord Tissue via Chemical Reprogramming[EB/OL].(2025-03-28)[2025-05-08].https://www.biorxiv.org/content/10.1101/2024.04.10.588154.点此复制
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