Multiplexed single-cell proteomics using SCoPE2
Multiplexed single-cell proteomics using SCoPE2
Abstract Many biological systems are composed of diverse single cells. This diversity necessitates functional and molecular single-cell analysis. Single-cell protein analysis has long relied on affinity reagents, but emerging mass-spectrometry methods (either label-free or multiplexed) have enabled quantifying over 1,000 proteins per cell while simultaneously increasing the specificity of protein quantification. Isobaric carrier based multiplexed single-cell proteomics is a scalable, reliable, and cost-effective method that can be fully automated and implemented on widely available equipment. It uses inexpensive reagents and is applicable to any sample that can be processed to a single-cell suspension. Here we describe an automated Single Cell ProtEomics (SCoPE2) workflow that allows analyzing about 200 single cells per 24 hours using only standard commercial equipment. We emphasize experimental steps and benchmarks required for achieving quantitative protein analysis. biorxiv;2021.03.12.435034v1/UFIG1F1ufig1
Petelski Aleksandra A.、Emmott Edward、Huffman R. Gray、Leduc Andrew、Perlman David H.、Specht Harrison、Slavov Nikolai
Department of Bioengineering, Northeastern University||Barnett Institute, Northeastern UniversityDepartment of Bioengineering, Northeastern University||Barnett Institute, Northeastern University||Centre for Proteome Research, Department of Biochemistry & Systems Biology, University of LiverpoolDepartment of Bioengineering, Northeastern University||Barnett Institute, Northeastern UniversityDepartment of Bioengineering, Northeastern University||Barnett Institute, Northeastern UniversityDepartment of Bioengineering, Northeastern University||Merck Exploratory Sciences CenterDepartment of Bioengineering, Northeastern University||Barnett Institute, Northeastern UniversityDepartment of Bioengineering, Northeastern University||Barnett Institute, Northeastern University||Department of Biology, Northeastern University
生物科学研究方法、生物科学研究技术细胞生物学生物化学
Petelski Aleksandra A.,Emmott Edward,Huffman R. Gray,Leduc Andrew,Perlman David H.,Specht Harrison,Slavov Nikolai.Multiplexed single-cell proteomics using SCoPE2[EB/OL].(2025-03-28)[2025-06-12].https://www.biorxiv.org/content/10.1101/2021.03.12.435034.点此复制
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