Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV
Alrubayyi Aljawharah 1Touizer Emma 2Kopycinski Jakub 1Roustan Chloe 3Waters Laura 4Burns Fiona 5Rowland-Jones Sarah 1McCoy Laura E. 2Charlton Bethany 1Kinloch Sabine 6Dorrell Lucy 1Gea-Mallorqu¨a Ester 1Muir Luke 2Hameiri-Bowen Dan 1Rosa Annachiara 3Peppa Dimitra 7Cherepanov Peter 3Fisher-Pearson Natasha 1Dong Tao 1Pellegrino Pierre 4Earl Christopher3
作者信息
- 1. Nuffield Dept of Clinical Medicine, University of Oxford
- 2. Division of Infection and Immunity, University College London
- 3. Chromatin Structure and Mobile DNA Laboratory, The Francis Crick Institute
- 4. Mortimer Market Centre, Department of HIV
- 5. Institute for Global Health UCL||Royal Free London NHS Foundation Trust
- 6. Royal Free London NHS Foundation Trust
- 7. Nuffield Dept of Clinical Medicine, University of Oxford||Mortimer Market Centre, Department of HIV
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Abstract
Abstract
There is an urgent need to understand the nature of immune responses generated against SARS-CoV-2, to better inform risk-mitigation strategies for people living with HIV (PLWH). Although not all PLWH are considered immunosuppressed, residual cellular immune deficiency and ongoing inflammation could influence COVID-19 disease severity, the evolution and durability of protective memory responses. Here, we performed an integrated analysis, characterizing the nature, breadth and magnitude of SARS-CoV-2-specific immune responses in PLWH, controlled on ART, and HIV negative subjects. Both groups were in the convalescent phase of predominately mild COVID-19 disease. The majority of PLWH mounted SARS-CoV-2 Spike- and Nucleoprotein-specific antibodies with neutralizing activity and SARS-CoV-2-specific T cell responses, as measured by ELISpot, at levels comparable to HIV negative subjects. T cell responses against Spike, Membrane and Nucleocapsid were the most prominent, with SARS-CoV-2-specific CD4 T cells outnumbering CD8 T cells. Notably, the overall magnitude of SARS-CoV-2-specific T cell responses related to the size of the naive CD4 T cell pool and the CD4:CD8 ratio in PLWH, in whom disparate antibody and T cell responses were observed. Both humoral and cellular responses to SARS-CoV-2 were detected at 5-7 months post-infection, providing evidence of medium-term durability of responses irrespective of HIV serostatus. Incomplete immune reconstitution on ART and a low CD4:CD8 ratio could, however, hamper the development of immunity to SARS-CoV-2 and serve as a useful tool for risk stratification of PLWH. These findings have implications for the individual management and potential effectiveness of vaccination against SARS-CoV-2 in PLWH.
One Sentence SummaryAdaptive immune responses to SARS-CoV-2 in the setting of HIV infection引用本文复制引用
Alrubayyi Aljawharah,Touizer Emma,Kopycinski Jakub,Roustan Chloe,Waters Laura,Burns Fiona,Rowland-Jones Sarah,McCoy Laura E.,Charlton Bethany,Kinloch Sabine,Dorrell Lucy,Gea-Mallorqu¨a Ester,Muir Luke,Hameiri-Bowen Dan,Rosa Annachiara,Peppa Dimitra,Cherepanov Peter,Fisher-Pearson Natasha,Dong Tao,Pellegrino Pierre,Earl Christopher.Characterization of humoral and SARS-CoV-2 specific T cell responses in people living with HIV[EB/OL].(2025-03-28)[2026-05-01].https://www.biorxiv.org/content/10.1101/2021.02.15.431215.学科分类
医药卫生理论/医学研究方法/基础医学
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