Clustering of desmosomal cadherins by desmoplakin is essential for cell-cell adhesion
Clustering of desmosomal cadherins by desmoplakin is essential for cell-cell adhesion
ABSTRACT Desmoplakin (Dp) localizes to desmosomes, linking clusters of desmosomal adhesion molecules to the intermediate filament cytoskeleton. Here, we generated Dp knockout (ko) cell lines of human keratinocytes to study the impact on desmosomal adhesion molecules and desmosome turnover using atomic force microscopy and superresolution imaging. In comparison to ko of another desmosomal component, plakoglobin (Pg), loss of Dp resulted in absence of desmosomes and drastically impaired cell cohesion. In Dp ko, the desmosomal adhesion molecules desmoglein 2 (Dsg2) and desmocollin 3 (Dsc3) were redistributed into small clusters in the cell membrane with no further increase in loss of intercellular adhesion by silencing of Dsg2. This suggests that extradesmosomal cadherins do not significantly contribute to cell cohesion but rather localization within desmosomes is required. Our data outline a crucial role of Dp for both desmosomal molecule clustering and mature desmosome formation and provide novel insights into the regulation of intercellular adhesion.
Waschke Jens、Brantschen Dominique、Walter Elias、Hiermaier Matthias、Wanuske Marie-Ther¨¨s、Schinner Camilla、St¨1dle Chiara、Vielmuth Franziska、Spindler Volker
Faculty of Medicine, Ludwig-Maximilians-Universit?t MunichDepartment of Biomedicine, University of BaselFaculty of Medicine, Ludwig-Maximilians-Universit?t MunichDepartment of Biomedicine, University of Basel||Faculty of Medicine, Ludwig-Maximilians-Universit?t MunichDepartment of Biomedicine, University of Basel||Faculty of Medicine, Ludwig-Maximilians-Universit?t MunichDepartment of Biomedicine, University of Basel||Faculty of Medicine, Ludwig-Maximilians-Universit?t MunichDepartment of Biomedicine, University of BaselFaculty of Medicine, Ludwig-Maximilians-Universit?t MunichDepartment of Biomedicine, University of Basel||Faculty of Medicine, Ludwig-Maximilians-Universit?t Munich
细胞生物学分子生物学
Waschke Jens,Brantschen Dominique,Walter Elias,Hiermaier Matthias,Wanuske Marie-Ther¨¨s,Schinner Camilla,St¨1dle Chiara,Vielmuth Franziska,Spindler Volker.Clustering of desmosomal cadherins by desmoplakin is essential for cell-cell adhesion[EB/OL].(2025-03-28)[2025-05-04].https://www.biorxiv.org/content/10.1101/2020.04.06.027185.点此复制
评论