Sequence and epigenetic landscapes of active and silenced nucleolus organizers in Arabidopsis
Sequence and epigenetic landscapes of active and silenced nucleolus organizers in Arabidopsis
Abstract Arabidopsis thaliana has two ribosomal RNA gene loci, nucleolus organizer regions NOR2 and NOR4, whose complete sequences remain unknown. Using ultra-long DNA sequencing technology, we generated 5.5 and 3.9 Mbp sequence assemblies for NOR2 and NOR4 (in the reference strain, Col-0), revealing their distinctive gene subtype compositions. RNA sequencing, and gene identification within flow-sorted nucleoli, in both wild-type and histone deacetylase 6 mutants, shows that most, but not all, NOR4 genes are active whereas most, but not all, NOR2 genes are epigenetically silenced. Long intervals of low CG and CHG methylation overlap regions of gene activity and gene subtype homogenization. Collectively, the data illuminate the genetic and epigenetic landscapes of the NORs and implicate transcription in rRNA gene concerted evolution. One Sentence SummarySequencing of Arabidopsis NORs reveals their organization, activity, and patterns of rRNA gene sequence homogenization.
McKinlay Anastasia、Enganti Ramya、Pikaard Craig S.、Fultz Dalen
Howard Hughes Medical Institute, Indiana University||Department of Biology, Indiana University||Department of Molecular and Cellular Biochemistry, Indiana UniversityHoward Hughes Medical Institute, Indiana University||Department of Biology, Indiana University||Department of Molecular and Cellular Biochemistry, Indiana UniversityHoward Hughes Medical Institute, Indiana University||Department of Biology, Indiana University||Department of Molecular and Cellular Biochemistry, Indiana UniversityHoward Hughes Medical Institute, Indiana University||Department of Biology, Indiana University||Department of Molecular and Cellular Biochemistry, Indiana University
遗传学分子生物学植物学
McKinlay Anastasia,Enganti Ramya,Pikaard Craig S.,Fultz Dalen.Sequence and epigenetic landscapes of active and silenced nucleolus organizers in Arabidopsis[EB/OL].(2025-03-28)[2025-05-05].https://www.biorxiv.org/content/10.1101/2023.06.07.544131.点此复制
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