Comparative analysis of early divergent land plants and construction of DNA tools for hyper-expression in Marchantia chloroplasts
Comparative analysis of early divergent land plants and construction of DNA tools for hyper-expression in Marchantia chloroplasts
ABSTRACT Chloroplast genes are present at high ploidy in plants, and capable of driving very high levels of gene expression if mRNA production and stability are properly regulated. Marchantia polymorpha is a simple model plant that allows rapid transformation studies, however post-transcriptional regulation in plastids is poorly characterized in this liverwort. We have mapped patterns of transcription in Marchantia chloroplasts. Furthermore, we have obtained and compared sequences from 51 early-divergent plant species, and identified putative sites for pentatricopeptide repeat protein binding that are thought to play important roles in mRNA stabilisation. Candidate binding sites were tested for their ability to confer high levels of reporter gene expression in Marchantia chloroplasts, and levels of protein production and effects on growth were measured in homoplasmic transformed plants. We have produced novel DNA tools for protein hyper-expression in a facile plant system that is a test-bed for chloroplast engineering.
Schneider Harald、Goffinet Bernard、Yu Ying、Haseloff Jim、Frangedakis Eftychios、Rebmann Marius、Liu Yang、Sauret-Gueto Susanna、Perraki Artemis、Tse Sze Wai、Guzman-Chavez Fernando、Markel Kasey、Rever Jenna
Center for Integrative Conservation, Xishuangbanna Tropical Botanical Garden, Chinese Academy of SciencesDepartment of Ecology and Evolutionary Biology, University of ConnecticutCollege of Life and Environmental Sciences, Hangzhou Normal UniversityDepartment of Plant Sciences, University of CambridgeDepartment of Plant Sciences, University of CambridgeDepartment of Plant Sciences, University of CambridgeFairy Lake Botanical Garden & Chinese Academy of SciencesDepartment of Plant Sciences, University of Cambridge||Crop Science Centre, University of CambridgeDepartment of Plant Sciences, University of Cambridge||Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - HellasDepartment of Plant Sciences, University of CambridgeDepartment of Plant Sciences, University of CambridgeDepartment of Plant Sciences, University of Cambridge||Department of Plant Biology, University of CaliforniaDepartment of Plant Sciences, University of Cambridge
分子生物学植物学生物工程学
Schneider Harald,Goffinet Bernard,Yu Ying,Haseloff Jim,Frangedakis Eftychios,Rebmann Marius,Liu Yang,Sauret-Gueto Susanna,Perraki Artemis,Tse Sze Wai,Guzman-Chavez Fernando,Markel Kasey,Rever Jenna.Comparative analysis of early divergent land plants and construction of DNA tools for hyper-expression in Marchantia chloroplasts[EB/OL].(2025-03-28)[2025-04-29].https://www.biorxiv.org/content/10.1101/2020.11.27.401802.点此复制
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