Genetic analysis of RNA polymerase I unveils new role of the Rpal2 subunit during transcription
Genetic analysis of RNA polymerase I unveils new role of the Rpal2 subunit during transcription
Summary Most transcriptional activity of exponentially growing cells is carried out by RNA Polymerase I (Pol I), which produces a large rRNA precursor. The Pol I transcription cycle is achieved through complex structural rearrangements of the enzyme, revealed by recent structural studies. In the yeast S. cerevisiae the Pol 1 subunit Rpa49, particularly its C-terminal tandem winged helix domain (Rpa49Ct), is required supports both initiation and elongation of the transcription cycle. Here, we characterized novel extragenic suppressors of the growth defect caused by the absence of Rpa49. We identified suppressor mutations on the two largest subunits of Pol I, Rpa190 and Rpa135, as well as Rpa12. Suppressor mutants RPA135-F301S and RPA12-S6L restored normal rRNA synthesis and increased Pol I density on rDNA genes in the absence of Rpa49Ct. Most mutated residues cluster at an interface formed by the jaw in Rpa190, the lobe in Rpa135, and subunit Rpa12 when mapped on the structure of Pol I. Our genetic data in S. cerevisiae suggest a new role for Rpa12 at the jaw/lobe interface during transcription cycle.
Calvo Olga、Pilsl Michael、Genty Titouan、Normand Christophe、Leger-Silvestre Isabelle、Chauvier Adrien、Audibert Sylvain、Tschochner Herbert、Fern¨￠ndez-Tornero Carlos、Darri¨¨re Tommy、Gadal Olivier、Dez Christophe
Instituto de Biolog¨aa Funcional y Gen¨?mica, IBFG-CSIC, Universidad de SalamancaUniversit?t Regensburg, Biochemie-Zentrum Regensburg (BZR)Laboratoire de Biologie Mol¨|culaire Eucaryote, Centre de Biologie Integrative (CBI), Universit¨| de Toulouse, CNRS, UPSLaboratoire de Biologie Mol¨|culaire Eucaryote, Centre de Biologie Integrative (CBI), Universit¨| de Toulouse, CNRS, UPSLaboratoire de Biologie Mol¨|culaire Eucaryote, Centre de Biologie Integrative (CBI), Universit¨| de Toulouse, CNRS, UPSLaboratoire de Biologie Mol¨|culaire Eucaryote, Centre de Biologie Integrative (CBI), Universit¨| de Toulouse, CNRS, UPSLaboratoire de Biologie Mol¨|culaire Eucaryote, Centre de Biologie Integrative (CBI), Universit¨| de Toulouse, CNRS, UPSUniversit?t Regensburg, Biochemie-Zentrum Regensburg (BZR)Centro de Investigaciones Biol¨?gicas, CSICLaboratoire de Biologie Mol¨|culaire Eucaryote, Centre de Biologie Integrative (CBI), Universit¨| de Toulouse, CNRS, UPSLaboratoire de Biologie Mol¨|culaire Eucaryote, Centre de Biologie Integrative (CBI), Universit¨| de Toulouse, CNRS, UPSLaboratoire de Biologie Mol¨|culaire Eucaryote, Centre de Biologie Integrative (CBI), Universit¨| de Toulouse, CNRS, UPS
遗传学分子生物学细胞生物学
Calvo Olga,Pilsl Michael,Genty Titouan,Normand Christophe,Leger-Silvestre Isabelle,Chauvier Adrien,Audibert Sylvain,Tschochner Herbert,Fern¨￠ndez-Tornero Carlos,Darri¨¨re Tommy,Gadal Olivier,Dez Christophe.Genetic analysis of RNA polymerase I unveils new role of the Rpal2 subunit during transcription[EB/OL].(2025-03-28)[2025-04-29].https://www.biorxiv.org/content/10.1101/307199.点此复制
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