DNA damage induced during mitosis undergoes DNA repair synthesis
DNA damage induced during mitosis undergoes DNA repair synthesis
Abstract Understanding the mitotic DNA damage response (DDR) is critical to our comprehension of cancer, premature aging and developmental disorders which are marked by DNA repair deficiencies. In this study we use a micro-focused-laser to induce DNA damage in selected mitotic chromosomes to study the subsequent repair response. Our findings demonstrate that (1) mitotic cells are capable of DNA repair as evidenced by DNA synthesis at damage sites, (2) Repair is attenuated when DNA-PKcs and ATM are simultaneously compromised, (3) Laser damage may permit the observation of previously undetected DDR proteins when damage is elicited by other methods in mitosis, and (4) Twenty five percent of mitotic DNA-damaged cells undergo a subsequent mitosis. Together these findings suggest that mitotic DDR is more complex than previously thought and may involve factors from multiple repair pathways that are better understood in interphase.
Berns Michael W.、Kabbara Sami、Sherman Adria、Cohen Shirli、Kong Xiangduo、Shi Zhixia、Preece Daryl、Godinez Veronica Gomez、Yokomori Kyoko、Maravillas-Montero Jose Luis、Wu Tao
Institute of Engineering in Medicine, University of Ca-San Diego||Department of Developmental and Cell Biology, University of Ca-Irvine||Beckman Laser Institute, University of Ca-Irvine||Department of Biomedical Engineering, University of Ca-IrvineDepartment of Developmental and Cell Biology, University of Ca-Irvine||Beckman Laser Institute, University of Ca-IrvineInstitute of Engineering in Medicine, University of Ca-San Diego||Beckman Laser Institute, University of Ca-IrvineInstitute of Engineering in Medicine, University of Ca-San DiegoDepartment of Biological Chemistry, University of Ca-IrvineInstitute of Engineering in Medicine, University of Ca-San DiegoDepartment of Biomedical Engineering, University of Ca-Irvine||Beckman Laser Institute, University of Ca-IrvineInstitute of Engineering in Medicine, University of Ca-San DiegoDepartment of Biological Chemistry, University of Ca-IrvineDepartment of Physiology, University of Ca-IrvineBeckman Laser Institute, University of Ca-Irvine||Department of Biomedical Engineering, University of Ca-Irvine
基础医学生物科学研究方法、生物科学研究技术分子生物学
Berns Michael W.,Kabbara Sami,Sherman Adria,Cohen Shirli,Kong Xiangduo,Shi Zhixia,Preece Daryl,Godinez Veronica Gomez,Yokomori Kyoko,Maravillas-Montero Jose Luis,Wu Tao.DNA damage induced during mitosis undergoes DNA repair synthesis[EB/OL].(2025-03-28)[2025-05-17].https://www.biorxiv.org/content/10.1101/2020.01.03.893784.点此复制
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