A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula
A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula
Abstract The retina, whose basic cellular structure is highly conserved across vertebrates, constitutes an accessible system for studying the central nervous system. In recent years, single-cell RNA-sequencing studies have uncovered cellular diversity in the retina of a variety of species, providing new insights on retinal evolution and development. However, similar data in cartilaginous fishes, the sister group to all other extant jawed vertebrates, are still lacking. Here, we present a single-nucleus RNA-sequencing atlas of the postnatal retina of the catshark Scyliorhinus canicula, consisting of the expression profiles for 17,438 individual cells from three female, juvenile catshark specimens. Unsupervised clustering revealed 22 distinct cell types comprising all major retinal cell classes, as well as retinal progenitor cells (whose presence reflects the persistence of proliferative activity in postnatal stages in sharks) and oligodendrocytes. Thus, our dataset serves as a foundation for further studies on the development and function of the catshark retina. Moreover, integration of our atlas with data from other species will allow for a better understanding of vertebrate retinal evolution.
Vidal-V¨￠zquez Nicol¨￠s、Carballo-Pacoret Pablo、Mazan Sylvie、Salisbury Sarah、Hervas-Sotomayor Francisca、Schmidt Julia、Adrio F¨￠tima、Robledo Diego、Candal Eva、Yuan Xuefei、Lamanna Francesco、Schneider C¨|line、Hern¨￠ndez-N¨2?ez Ismael、Villamayor Paula R.、Kaessmann Henrik、Barreiro-Iglesias Ant¨?n
Departamento de Biolox¨aa Funcional, Facultade de Biolox¨aa, Universidade de Santiago de Compostela||Aquatic One Health Research Center (ARCUS), Universidade de Santiago de CompostelaDepartamento de Biolox¨aa Funcional, Facultade de Biolox¨aa, Universidade de Santiago de Compostela||The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of EdinburghCNRS, Sorbonne Universit¨|, Biologie Int¨|grative des Organismes Marins, UMR7232-BIOMThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of EdinburghCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg University||INRAE, LPGPCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityDepartamento de Biolox¨aa Funcional, Facultade de Biolox¨aa, Universidade de Santiago de CompostelaThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh||Departamento de Zoolox¨aa, Xen¨|tica e Antropolox¨aa F¨asica, Facultade de Biolox¨aa, Universidade de Santiago de CompostelaDepartamento de Biolox¨aa Funcional, Facultade de Biolox¨aa, Universidade de Santiago de Compostela||Aquatic One Health Research Center (ARCUS), Universidade de Santiago de CompostelaCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityDepartamento de Biolox¨aa Funcional, Facultade de Biolox¨aa, Universidade de Santiago de CompostelaThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh||Departamento de Zoolox¨aa, Xen¨|tica e Antropolox¨aa F¨asica, Facultade de Veterinaria, Universidade de Santiago de CompostelaCenter for Molecular Biology (ZMBH), DKFZ-ZMBH Alliance, Heidelberg UniversityDepartamento de Biolox¨aa Funcional, Facultade de Biolox¨aa, Universidade de Santiago de Compostela||Aquatic One Health Research Center (ARCUS), Universidade de Santiago de Compostela
细胞生物学遗传学分子生物学
Vidal-V¨￠zquez Nicol¨￠s,Carballo-Pacoret Pablo,Mazan Sylvie,Salisbury Sarah,Hervas-Sotomayor Francisca,Schmidt Julia,Adrio F¨￠tima,Robledo Diego,Candal Eva,Yuan Xuefei,Lamanna Francesco,Schneider C¨|line,Hern¨￠ndez-N¨2?ez Ismael,Villamayor Paula R.,Kaessmann Henrik,Barreiro-Iglesias Ant¨?n.A single-nucleus RNA sequencing atlas of the postnatal retina of the shark Scyliorhinus canicula[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2024.04.12.589211.点此复制
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