FRUITFULL-like genes regulate flowering time and inflorescence architecture in tomato
FRUITFULL-like genes regulate flowering time and inflorescence architecture in tomato
ABSTRACT The timing of flowering and inflorescence architecture are critical for the reproductive success of tomato, but the gene regulatory networks underlying these traits have not been fully explored. Here we show that the tomato FRUITFULL-like (FUL-like) genes FUL2 and MADS-BOX PROTEIN 20 (MBP20) induce flowering and repress inflorescence branching by promoting floral meristem determinacy. FUL1 fulfils a less prominent role and appears to depend on FUL2 and MBP20 for its upregulation in the inflorescence- and floral meristems. MBP10, the fourth tomato FUL-like gene, has probably lost its function. The tomato FUL-like proteins cannot homodimerize in in vitro assays, but heterodimerize with various other MADS-domain proteins, potentially forming distinct complexes in the transition meristem and floral meristem. Transcriptome analysis of the primary shoot meristems revealed various interesting downstream targets, including four repressors of cytokinin signalling that are upregulated during the vegetative-to-reproductive transition in ful1 ful2 mbp10 mbp20 mutants. FUL2 and MBP20 can also bind in vitro to the upstream regions of these genes, thereby probably directly stimulating cell division in the meristem upon the transition to flowering. The control of inflorescence branching does not occur via the CKXs, but appears regulated by repression of transcription factors such as TM3, APETALA 2b (AP2b) and AP2a.
Lubini Greice、Jiang Xiaobing、Hernandes-Lopes Jos¨|、Rijnsburger Kim、de Maagd Ruud A.、Angenent Gerco C.、Veltkamp Vera、Bemer Marian
Business Unit Bioscience, Wageningen University & Research||Departamento de Biologia, Faculdade de Filosofia, Ci¨oncias e Letras de Ribeir?o Preto, Universidade de S?o Paulo||PPG-Gen¨|tica, Faculdade de Medicina de Ribeir?o Preto, Universidade de S?o PauloLaboratory of Molecular Biology, Wageningen University & Research||Business Unit Bioscience, Wageningen University & ResearchBusiness Unit Bioscience, Wageningen University & Research||Departamento de Botanica, Instituto de Bioci¨oncias, Universidade de S?o PauloLaboratory of Molecular Biology, Wageningen University & ResearchBusiness Unit Bioscience, Wageningen University & ResearchLaboratory of Molecular Biology, Wageningen University & Research||Business Unit Bioscience, Wageningen University & ResearchLaboratory of Molecular Biology, Wageningen University & Research||Business Unit Bioscience, Wageningen University & ResearchLaboratory of Molecular Biology, Wageningen University & Research||Business Unit Bioscience, Wageningen University & Research
分子生物学植物学遗传学
Lubini Greice,Jiang Xiaobing,Hernandes-Lopes Jos¨|,Rijnsburger Kim,de Maagd Ruud A.,Angenent Gerco C.,Veltkamp Vera,Bemer Marian.FRUITFULL-like genes regulate flowering time and inflorescence architecture in tomato[EB/OL].(2025-03-28)[2025-05-03].https://www.biorxiv.org/content/10.1101/2020.09.28.316562.点此复制
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