不同区域脑组织对运动诱导氧化应激和DNA损伤的反应

Objective: To inverstigate the effects of 8 weeks swimming training on oxidative stress system and DNA damage in different brain region in rats. Method: The animals were divided into the following categories: adult control (4 months), adult swimming exercise (4 months). Twenty-four rats were placed in the tank for swimming twice a day for 90min, each for a total of 8 weeks. In rat special brain levels of MDA and GPx were measured. Furthermore, the expression of 8-OHdG was detected with immunohistochemical method. Result: Here we showed that 8 weeks swimming training resulted in increased GPx activity in the rat hippocampus accompanied by significant decreased in baseline MDA levels. Interestingly, there was no significant difference in the baseline MDA levels and the GPx values in rat cortex.Meanwhile, the comparison of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in both the hippocampus and the cortex did not reveal any significant differences. Conclution：This swimming protocol appears to increase the activity of GPx only in the hippocampus, that do not happen in the cortex. Although exercise training affects the activity of GPx in hippocampus and cortex differently, exercise do not increase DNA damage in the cortex. These data provide evidence for differential regulation dependent on brain region in oxidative challenge-associated adaptation during exercise.