Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy
Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy
Abstract Brillouin microscopy is a promising all-optical technique for biomechanics but is limited by slow acquisition speed and/or large irradiation doses. Here, we introduce multiplexed Brillouin microscopy that overcomes both these limits by over one order of magnitude with selective illumination and single-shot analysis of hundreds of points along the incident beam axis. We demonstrate the enabling capabilities of this method probing rapid response to perturbations and long-term mechanical evolution of tumor spheroids.
Zhang Jitao、Nikolic Milos、Scarcelli Giuliano、Tanner Kandice
Fischell Department of Bioengineering, University of Maryland||Department of Biomedical Engineering, Wayne State UniversityFischell Department of Bioengineering, University of Maryland||Maryland Biophysics Program, University of Maryland||Laboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of HealthFischell Department of Bioengineering, University of Maryland||Maryland Biophysics Program, University of MarylandLaboratory of Cell Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health
生物科学研究方法、生物科学研究技术生物物理学基础医学
Zhang Jitao,Nikolic Milos,Scarcelli Giuliano,Tanner Kandice.Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy[EB/OL].(2025-03-28)[2025-06-20].https://www.biorxiv.org/content/10.1101/2022.04.25.489096.点此复制
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