An online atlas of human plasma metabolite signatures of gut microbiome composition
An online atlas of human plasma metabolite signatures of gut microbiome composition
Summary paragraph The human gut microbiota produces a variety of small compounds, some of which enter the bloodstream and impact host health. Conversely, various exogenous nutritional and pharmaceutical compounds affect the gut microbiome composition before entering circulation. Characterization of the gut microbiota–host plasma metabolite interactions is an important step towards understanding the effects of the gut microbiota on human health. However, studies involving large and deeply phenotyped cohorts that would reveal such meaningful interactions are scarce. Here, we used deep metagenomic sequencing and ultra-high-performance liquid chromatography linked to mass spectrometry for detailed characterization of the fecal microbiota and plasma metabolome, respectively, of 8,584 participants invited at age 50 to 64 of the Swedish CArdioPulmonary bioImage Study (SCAPIS). After adjusting for multiple comparisons, we identified 1,008 associations between species alpha diversity and plasma metabolites, and 318,944 associations between specific gut metagenomic species and plasma metabolites. The gut microbiota explained up to 50% of the variance of individual plasma metabolites (mean of 4.7%). We present all results as the searchable association atlas “GUTSY” as a rich resource for mining associations, and exemplify the potential of the atlas by presenting novel associations between oral medication and the gut microbiome, and microbiota species strongly associated with levels of the uremic toxin p-cresol sulfate. The association atlas can be used as the basis for targeted studies of perturbation of specific bacteria and for identification of candidate plasma biomarkers of gut flora composition.
Fall Tove、Dekkers Koen F.、Nowak Christoph、Varotsis Georgios、Brunkwall Louise、Eklund Aron C.、Holm Jacob Bak、Ottosson Filip、Lind Lars、Sundstr?m Johan、Orho-Melander Marju、Smith J. Gustav、Nguyen Diem、Sayols-Baixeras Sergi、Lin Yi-Ting、Engstr?m Gunnar、Baldanzi Gabriel、Hammar Ulf、Nielsen Nynne、Nielsen H. Bj?rn、?rnl?v Johan、Ahmad Shafqat
Department of Medical Sciences, Molecular Epidemiology and Science for Life Laboratory, Uppsala UniversityDepartment of Medical Sciences, Molecular Epidemiology and Science for Life Laboratory, Uppsala UniversityDivision of Family Medicine and Primary Care, Department of Neurobiology, Care Science and Society, Karolinska InstituteDepartment of Medical Sciences, Molecular Epidemiology and Science for Life Laboratory, Uppsala UniversityDepartment of Clinical Sciences, Lund UniversityClinical Microbiomics A/SClinical Microbiomics A/SDepartment of Clinical Sciences, Lund UniversityDepartment of Medical Sciences, Uppsala UniversityDepartment of Medical Sciences, Clinical Epidemiology, Uppsala University||The George Institute for Global Health, University of New South WalesDepartment of Clinical Sciences, Lund UniversityThe Wallenberg Laboratory/Department of Molecular and Clinical Medicine, Institute of Medicine, Gothenburg University and the Department of Cardiology, Sahlgrenska University Hospital||Department of Cardiology, Clinical Sciences, Lund University and Sk?ne University Hospital||Wallenberg Center for Molecular Medicine and Lund University Diabetes Center, Lund UniversityDepartment of Medical Sciences, Molecular Epidemiology and Science for Life Laboratory, Uppsala UniversityDepartment of Medical Sciences, Molecular Epidemiology and Science for Life Laboratory, Uppsala UniversityDepartment of Medical Sciences, Molecular Epidemiology and Science for Life Laboratory, Uppsala UniversityDepartment of Clinical Sciences, Lund UniversityDepartment of Medical Sciences, Molecular Epidemiology and Science for Life Laboratory, Uppsala UniversityDepartment of Medical Sciences, Molecular Epidemiology and Science for Life Laboratory, Uppsala UniversityClinical Microbiomics A/SClinical Microbiomics A/SDivision of Family Medicine and Primary Care, Department of Neurobiology, Care Science and Society, Karolinska Institute||School of Health and Social Studies, Dalarna UniversityDepartment of Medical Sciences, Molecular Epidemiology and Science for Life Laboratory, Uppsala University
基础医学微生物学生物化学
Fall Tove,Dekkers Koen F.,Nowak Christoph,Varotsis Georgios,Brunkwall Louise,Eklund Aron C.,Holm Jacob Bak,Ottosson Filip,Lind Lars,Sundstr?m Johan,Orho-Melander Marju,Smith J. Gustav,Nguyen Diem,Sayols-Baixeras Sergi,Lin Yi-Ting,Engstr?m Gunnar,Baldanzi Gabriel,Hammar Ulf,Nielsen Nynne,Nielsen H. Bj?rn,?rnl?v Johan,Ahmad Shafqat.An online atlas of human plasma metabolite signatures of gut microbiome composition[EB/OL].(2025-03-28)[2025-08-29].https://www.medrxiv.org/content/10.1101/2021.12.23.21268179.点此复制
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