Evolution of the bread wheat D-subgenome and enriching it with diversity from Aegilops tauschii
Evolution of the bread wheat D-subgenome and enriching it with diversity from Aegilops tauschii
Abstract Aegilops tauschii, the diploid wild progenitor of the D-subgenome of bread wheat, constitutes a reservoir of genetic diversity for improving bread wheat performance and environmental resilience. To better define and understand this diversity, we sequenced 242 Ae. tauschii accessions and compared them to the wheat D-subgenome. We characterized a rare, geographically-restricted lineage of Ae. tauschii and discovered that it contributed to the wheat D-subgenome, thereby elucidating the origin of bread wheat from at least two independent hybridizations. We then used k-mer-based association mapping to identify discrete genomic regions with candidate genes for disease and pest resistance and demonstrated their functional transfer into wheat by transgenesis and wide crossing, including the generation of a library of ‘synthetic’ hexaploids incorporating diverse Ae. tauschii genomes. This pipeline permits rapid trait discovery in the diploid ancestor through to functional genetic validation in a hexaploid background amenable to breeding.
Pearce Suzi、Lagudah Evans、Kirana Rizky Pasthika、Mehrabi Ali A.、Steffenson Brian J.、Elkot Ahmed F.、Davey Robert P.、Mao Long、Brown-Guedira Gina、Lux Thomas、Gaurav Kumar、Arora Sanu、Gao Liangliang、Nicholson Paul、Quiroz-Ch¨￠vez Jes¨2s、Smedley Mark A.、Gilbert David、Forner-Mart¨anez Macarena、Liu Jiaqian、Hafeez Amber N.、O?ˉHara Tom、Justesen Annemarie Fejer、Avni Raz、Buerstmayr Hermann、Tyrrell Simon、Uauy Cristobal、Mascher Martin、Poland Jesse、Wulff Brande B. H.、Cheema Jitender、Chhuneja Parveen、Liu Deng-Cai、Sharon Amir、Simonsen Martin、Yu Guotai、Steuernagel Burkhard、Steiner Barbara、Rudd Jackie C.、Hayta Sadiye、Brar Gurcharn S.、S¨￠nchez-Mart¨an Javier、Bentley Alison R.、Simmonds James、Keller Beat、Mayer Klaus F. X.、Kutcher H. Randy、Gardener Catherine、Artmeier Susanne、Chayut Noam、Hucl Pierre、Schauser Leif、Waites Joshua、Raupp Jon、Horsnell Richard、Xu Steven、Harwood Wendy、Luo Ming-Cheng、Kale Sandip M.、Pascucci Attilio、Boden Scott、Krasileva Ksenia、Nasyrova Firuza Y.、Singh Narinder、Sandhu Nitika、Matny Oadi、Chinoy Catherine、Kangara Ngonidzashe、Li Aili、Liu Shuyu、Silva Paula、Widrig Victoria、Faris Justin、Wu Shuangye、Patpour Mehran、Dvorak Jan、Ghosh Sreya、Bian Xingdong、Tiwari Vijay K.
John Innes Centre, Norwich Research ParkCommonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and FoodInstitute of Biotechnology in Plant Production, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences||Laboratory of Plant Breeding, Department of Agronomy, Faculty of Agriculture, Universitas Gadjah MadaDepartment of Agronomy and Plant Breeding, Ilam UniversityDepartment of Plant Pathology, University of MinnesotaWheat Research Department, Field Crops Research Institute, Agricultural Research CenterEarlham Institute, Norwich Research ParkInstitute of Crop Science, Chinese Academy of Agricultural SciencesUSDA-ARS, North Carolina State UniversityPlant Genome and Systems Biology, Helmholtz Center MunichJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research ParkDepartment of Plant Pathology and Wheat Genetics Resource Center, Kansas State UniversityJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research Park||National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/JCIC-MCPJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research ParkAarhus University, Department of Agroecology, Global Rust Reference CenterInstitute for Cereal Crops Improvement, School of Plant Sciences and Food Security, Tel Aviv UniversityInstitute of Biotechnology in Plant Production, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life SciencesEarlham Institute, Norwich Research ParkJohn Innes Centre, Norwich Research ParkLeibniz-Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben||German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-LeipzigDepartment of Plant Pathology and Wheat Genetics Resource Center, Kansas State UniversityJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research ParkSchool of Agricultural Biotechnology, Punjab Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityInstitute for Cereal Crops Improvement, School of Plant Sciences and Food Security, Tel Aviv UniversityQIAGEN Aarhus A/S, Silkeborgvej 2, AarhusJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research ParkInstitute of Biotechnology in Plant Production, Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life SciencesTexas A&M AgriLife Research, AmarilloJohn Innes Centre, Norwich Research ParkCrop Development Centre, Department of Plant Sciences, University of Saskatchewan||Faculty of Land and Food Systems, The University of British ColumbiaUniversity of Zurich, Department of Plant and Microbial BiologyThe John Bingham Laboratory||International Maize and Wheat Improvement Center (CIMMYT)John Innes Centre, Norwich Research ParkUniversity of Zurich, Department of Plant and Microbial BiologyPlant Genome and Systems Biology, Helmholtz Center Munich||Faculty of Life Sciences, Technical University MunichCrop Development Centre, Department of Plant Sciences, University of SaskatchewanJohn Innes Centre, Norwich Research ParkPlant Genome and Systems Biology, Helmholtz Center MunichGermplasm Resources Unit, John Innes Centre, NorwichCrop Development Centre, Department of Plant Sciences, University of SaskatchewanQIAGEN Aarhus A/S, Silkeborgvej 2, AarhusJohn Innes Centre, Norwich Research ParkDepartment of Plant Pathology and Wheat Genetics Resource Center, Kansas State UniversityThe John Bingham Laboratory||International Maize and Wheat Improvement Center (CIMMYT)USDA-ARS Cereal Crops Research Unit, Edward T. Schafer Agricultural Research CenterJohn Innes Centre, Norwich Research ParkDepartment of Plant Sciences, University of CaliforniaLeibniz-Institute of Plant Genetics and Crop Plant Research (IPK) GaterslebenJohn Innes Centre, Norwich Research Park||Department of Agricultural and Food Sciences, Alma Mater Studiorum, University of BolognaJohn Innes Centre, Norwich Research Park||School of Agriculture, Food and Wine, University of AdelaideDepartment of Plant and Microbial Biology, University of California, BerkeleyInstitute of Botany, Plant Physiology and Genetics, Tajik National Academy of SciencesDepartment of Plant Pathology and Wheat Genetics Resource Center, Kansas State University||Bayer R&D Services LLCSchool of Agricultural Biotechnology, Punjab Agricultural UniversityDepartment of Plant Pathology, University of MinnesotaJohn Innes Centre, Norwich Research ParkJohn Innes Centre, Norwich Research ParkInstitute of Crop Science, Chinese Academy of Agricultural SciencesTexas A&M AgriLife Research, AmarilloDepartment of Plant Pathology and Wheat Genetics Resource Center, Kansas State University||Instituto Nacional de Investigaci¨?n Agropecuaria (INIA)University of Zurich, Department of Plant and Microbial BiologyUSDA-ARS Cereal Crops Research Unit, Edward T. Schafer Agricultural Research CenterDepartment of Plant Pathology and Wheat Genetics Resource Center, Kansas State UniversityAarhus University, Department of Agroecology, Global Rust Reference CenterDepartment of Plant Sciences, University of CaliforniaJohn Innes Centre, Norwich Research ParkEarlham Institute, Norwich Research ParkDepartment of Plant Science and Landscape Architecture, University of Maryland, College Park
农业科学技术发展农业科学研究植物学
Pearce Suzi,Lagudah Evans,Kirana Rizky Pasthika,Mehrabi Ali A.,Steffenson Brian J.,Elkot Ahmed F.,Davey Robert P.,Mao Long,Brown-Guedira Gina,Lux Thomas,Gaurav Kumar,Arora Sanu,Gao Liangliang,Nicholson Paul,Quiroz-Ch¨￠vez Jes¨2s,Smedley Mark A.,Gilbert David,Forner-Mart¨anez Macarena,Liu Jiaqian,Hafeez Amber N.,O?ˉHara Tom,Justesen Annemarie Fejer,Avni Raz,Buerstmayr Hermann,Tyrrell Simon,Uauy Cristobal,Mascher Martin,Poland Jesse,Wulff Brande B. H.,Cheema Jitender,Chhuneja Parveen,Liu Deng-Cai,Sharon Amir,Simonsen Martin,Yu Guotai,Steuernagel Burkhard,Steiner Barbara,Rudd Jackie C.,Hayta Sadiye,Brar Gurcharn S.,S¨￠nchez-Mart¨an Javier,Bentley Alison R.,Simmonds James,Keller Beat,Mayer Klaus F. X.,Kutcher H. Randy,Gardener Catherine,Artmeier Susanne,Chayut Noam,Hucl Pierre,Schauser Leif,Waites Joshua,Raupp Jon,Horsnell Richard,Xu Steven,Harwood Wendy,Luo Ming-Cheng,Kale Sandip M.,Pascucci Attilio,Boden Scott,Krasileva Ksenia,Nasyrova Firuza Y.,Singh Narinder,Sandhu Nitika,Matny Oadi,Chinoy Catherine,Kangara Ngonidzashe,Li Aili,Liu Shuyu,Silva Paula,Widrig Victoria,Faris Justin,Wu Shuangye,Patpour Mehran,Dvorak Jan,Ghosh Sreya,Bian Xingdong,Tiwari Vijay K..Evolution of the bread wheat D-subgenome and enriching it with diversity from Aegilops tauschii[EB/OL].(2025-03-28)[2025-04-26].https://www.biorxiv.org/content/10.1101/2021.01.31.428788.点此复制
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