Desert Dingo ( Canis lupus dingo ) genome provides insights into their role in the Australian ecosystem
Yadav Sonu 1Aiden Erez L. 2Edwards Richard J. 1Manandhar Bikash 3Bustamante Sonia 4Rasmussen Jacob Agerbo 5Omer Arina 6Colaric Zane 6Minoche Andre E. 7Smith Timothy P.L. 8Zammit Robert A. 9Dudchenko Olga 10Thomas Torsten 11Bogdanovic Ozren 12Esvaran Meera 11Melvin Richard G. 13Chan Eva K. F. 14Rosen Benjamin D. 15Gopalakrishnan Shyam 16Skvortsova Ksenia 17Gilbert M. Thomas P. 18Cochran Blake J. 3Ballard J. William O. Field Matt A. 19Keilwagen Jens 20Bucknall Martin4
作者信息
- 1. School of Biotechnology and Biomolecular Sciences, UNSW
- 2. The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine||Department of Computer Science, Rice University||Center for Theoretical and Biological Physics, Rice University||Faculty of Science, UWA School of Agriculture and Environment, University of Western Australia||Shanghai Institute for Advanced Immunochemical Studies, Shanghai Tech University
- 3. School of Medical Sciences, University of New South Wales
- 4. Mark Wainwright Analytical Center, University of New South Wales
- 5. Center for Evolutionary Hologenomics, Faculty of Health and Medical Sciences, The GLOBE Institute University of Copenhagen||Laboratory of Genomics and Molecular Biomedicine, Department of Biology, University of Copenhagen
- 6. The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine
- 7. Garvan Institute of Medical Research
- 8. US Meat Animal Research Center, Agricultural Research Service USDA
- 9. Vineyard Veterinary Hospital
- 10. The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine||Department of Computer Science, Rice University||Center for Theoretical and Biological Physics, Rice University
- 11. School of Biological, Earth and Environmental Sciences, University of New South Wales
- 12. School of Biotechnology and Biomolecular Sciences, UNSW||Garvan Institute of Medical Research
- 13. Department of Biomedical Sciences, 1035 University Drive Duluth, University of Minnesota
- 14. NSW Health Pathology
- 15. Animal Genomics and Improvement Laboratory, Agricultural Research Service USDA
- 16. Center for Evolutionary Hologenomics, Faculty of Health and Medical Sciences, The GLOBE Institute University of Copenhagen
- 17. Garvan Institute of Medical Research||St Vincent?ˉs Clinical School, Faculty of Medicine, University of New South Wales
- 18. Center for Evolutionary Hologenomics, Faculty of Health and Medical Sciences, The GLOBE Institute University of Copenhagen||NTNU University Museum
- 19. Centre for Tropical Bioinformatics and Molecular Biology, Australian Institute of Tropical Health and Medicine, James Cook University||John Curtin School of Medical Research, Australian National University
- 20. Julius K¨1hn-Institut
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Abstract
Abstract
The dingo is Australia’s iconic top-order predator and arrived on the continent between 5,000-8,000 years ago. To provide an unbiased insight into its evolutionary affiliations and biological interactions, we coupled long-read DNA sequencing with a multiplatform scaffolding approach to produce an ab initio genome assembly of the desert dingo (85X coverage) we call CanLup_DDS. We compared this genome to the Boxer (CanFam3.1) and German Shepherd dog (CanFam_GSD) assemblies and characterized lineage-specific and shared genetic variation ranging from single– to megabase pair–sized variants. We identified 21,483 dingo-specific and 16,595 domestic dog-specific homozygous structural variants mediating genic and putative regulatory changes. Comparisons between the dingo and domestic dog builds detected unique inversions on Chromosome 16, structural variations in genes linked with starch metabolism, and seven differentially methylated genes. To experimentally assess genomic differences 17 dingoes and 15 German Shepherd dogs were fed parallel diets for 14 days. In dingoes, low AMY2B copy number and serum amylase levels are linked with high cholesterol and LDL levels. Gut microbiome analyses revealed enrichment of the family Clostridiaceae, which can utilize complex resistant starch, while scat metabolome studies identified high phenylethyl alcohol concentrations that we posit are linked with territory marking. Our study provides compelling genomic, microbiome, and metabolomic links showing the dingo has distinct physiology from domestic breed dogs with a unique role in the ecosystem.引用本文复制引用
Yadav Sonu,Aiden Erez L.,Edwards Richard J.,Manandhar Bikash,Bustamante Sonia,Rasmussen Jacob Agerbo,Omer Arina,Colaric Zane,Minoche Andre E.,Smith Timothy P.L.,Zammit Robert A.,Dudchenko Olga,Thomas Torsten,Bogdanovic Ozren,Esvaran Meera,Melvin Richard G.,Chan Eva K. F.,Rosen Benjamin D.,Gopalakrishnan Shyam,Skvortsova Ksenia,Gilbert M. Thomas P.,Cochran Blake J.,Ballard J. William O.,Field Matt A.,Keilwagen Jens,Bucknall Martin.Desert Dingo ( Canis lupus dingo ) genome provides insights into their role in the Australian ecosystem[EB/OL].(2025-03-28)[2026-04-03].https://www.biorxiv.org/content/10.1101/2020.11.15.384057.学科分类
生物科学现状、生物科学发展/环境生物学/动物学
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