LY-CoV555, a rapidly isolated potent neutralizing antibody, provides protection in a non-human primate model of SARS-CoV-2 infection
LY-CoV555, a rapidly isolated potent neutralizing antibody, provides protection in a non-human primate model of SARS-CoV-2 infection
Abstract SARS-CoV-2 poses a public health threat for which therapeutic agents are urgently needed. Herein, we report that high-throughput microfluidic screening of antigen-specific B-cells led to the identification of LY-CoV555, a potent anti-spike neutralizing antibody from a convalescent COVID-19 patient. Biochemical, structural, and functional characterization revealed high-affinity binding to the receptor-binding domain, ACE2 binding inhibition, and potent neutralizing activity. In a rhesus macaque challenge model, prophylaxis doses as low as 2.5 mg/kg reduced viral replication in the upper and lower respiratory tract. These data demonstrate that high-throughput screening can lead to the identification of a potent antiviral antibody that protects against SARS-CoV-2 infection. One Sentence SummaryLY-CoV555, an anti-spike antibody derived from a convalescent COVID-19 patient, potently neutralizes SARS-CoV-2 and protects the upper and lower airways of non-human primates against SARS-CoV-2 infection.
Higgs Richard E.、Hsieh Ching-Lin、Heinz Beverly A.、Hwang Yuri、?entelis Stefanie、Jepson Kevin R.、Goya Rodrigo、Smith Maia A.、Hinshaw Samuel J.、Tycho Sean A.、Muthuraman Krithika、Sobhanifar Solmaz、Adams Andrew C.、Berens Shawn J.、Geisbert Thomas W.、Belli Hayley M.、de Vries Maren、Mohamed Adil、Samanovic Marie、Johnson Nicole V.、Wiethoff Christopher M.、Barnhart Bryan C.、Mascola John R.、Borisevich Viktoriya、Brown-Augsburger Patricia L.、Xiang Ping、Pustilnik Anna、Graham Barney S.、Collins David W.、Triana Franz J.、Goldsmith Jory A.、Dittmann Meike、Jones Bryan E.、Kraft Lucas、Corbett Kizzmekia S.、Hendle Jorg、Bidshahri Roza、Davies Julian、Hansen Carl L.、Pellacani Davide、Abiona Olubukola、Balasubramaniam Deepa、Blackbourne Jamie L.、Mulligan Mark J.、Cross Robert W.、Foster Denisa、Piper Marissa H.、Westendorf Kathryn、Wang Lingshu、Baric Ralph S.、Cujec Thomas P.、Martinez David R.、Wrapp Daniel、McLellan Jason S.、Falconer Ester
医药卫生理论医学研究方法生物科学研究方法、生物科学研究技术
Higgs Richard E.,Hsieh Ching-Lin,Heinz Beverly A.,Hwang Yuri,?entelis Stefanie,Jepson Kevin R.,Goya Rodrigo,Smith Maia A.,Hinshaw Samuel J.,Tycho Sean A.,Muthuraman Krithika,Sobhanifar Solmaz,Adams Andrew C.,Berens Shawn J.,Geisbert Thomas W.,Belli Hayley M.,de Vries Maren,Mohamed Adil,Samanovic Marie,Johnson Nicole V.,Wiethoff Christopher M.,Barnhart Bryan C.,Mascola John R.,Borisevich Viktoriya,Brown-Augsburger Patricia L.,Xiang Ping,Pustilnik Anna,Graham Barney S.,Collins David W.,Triana Franz J.,Goldsmith Jory A.,Dittmann Meike,Jones Bryan E.,Kraft Lucas,Corbett Kizzmekia S.,Hendle Jorg,Bidshahri Roza,Davies Julian,Hansen Carl L.,Pellacani Davide,Abiona Olubukola,Balasubramaniam Deepa,Blackbourne Jamie L.,Mulligan Mark J.,Cross Robert W.,Foster Denisa,Piper Marissa H.,Westendorf Kathryn,Wang Lingshu,Baric Ralph S.,Cujec Thomas P.,Martinez David R.,Wrapp Daniel,McLellan Jason S.,Falconer Ester.LY-CoV555, a rapidly isolated potent neutralizing antibody, provides protection in a non-human primate model of SARS-CoV-2 infection[EB/OL].(2025-03-28)[2025-06-09].https://www.biorxiv.org/content/10.1101/2020.09.30.318972.点此复制
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