尾悬吊大鼠股直肌废用性肌萎缩的蛋白组学分析

Objective: To identify major muscle proteins responding significantly to the unloading, Compared with unsuspended controls, we examined protein expression in the rat muscle during 2-week hindlimb suspension by 2-DE. Methods: Establish the model of hindlimb suspension, to make rat unloading, then examined the proteins by 2-DE after 2-week. Result: we found 23 different expression proteins during unloading. Among these, we observed 18 decreased, and 5 increased. Conclusions: After 2-week hindlimb suspension, These results of unloading of skeletal muscle has a decrease change overall contractile proteins, cytoskeleton protein, transport proteins, but the metabolic proteins has both increase and decrease, it imply that the metabolic regulation is a complex process.