Quantitative Restoration of Immune Defense in Old Animals Determined by Na?ve Antigen-Specific CD8 T cell Numbers
Quantitative Restoration of Immune Defense in Old Animals Determined by Na?ve Antigen-Specific CD8 T cell Numbers
SUMMARY Older humans and animals often exhibit reduced immune responses to infection and vaccination, and this often directly correlates to the numbers and frequency of na?ve T (Tn) cells. We found such a correlation between reduced numbers of blood CD8+ Tn cells and severe clinical outcomes of WNV in both humans naturally exposed to, and mice experimentally infected with, West Nile virus (WNV). To examine possible causality, we sought to increase the number of CD8 Tn cells by treating C57BL/6 mice with IL-7 complexes (IL-7C, anti-IL-7 mAb bound to IL-7), shown previously to efficiently increase peripheral T cell numbers by homeostatic proliferation. T cells underwent robust expansion following IL-7C administration to old mice increasing the number of total T cells (>four-fold) and NS4b:H-2Db-restricted antigen-specific CD8 T cells (two-fold). This improved the numbers of NS4b-specific CD8 T cells detected at the peak of the response against WNV, but not survival in the face of WNV challenge. IL-7C treated old animals also showed no improvement in WNV-specific effector immunity (neutralizing antibody and in vivo T cell cytotoxicity). To test quantitative limits to which CD8 Tn cell restoration could improve protective immunity, we transferred graded doses of Ag-specific precursors into old mice and showed that injection of 5,400 (but not of 1,800 or 600) adult na?ve WNV-specific CD8 T cells significantly increased survival after WNV. These results set quantitative limits to the level of Tn reconstitution necessary to improve immune defense in older organisms and are discussed in light of targets of immune reconstitution.
Nikolich-?ugich Janko、Bradshaw Christine M.、Coplen Christopher P.、Dudakov Jarrod、Uhrlaub Jennifer L.、Lanteri Marion C.、Murray Kristy O.、van den Brink Marcel R. M.、Jergovi? Mladen、Busch Michael P.、Sonar Sandip
Department of Immunobiology, University of Arizona College of Medicine||University of Arizona Center on Aging, University of Arizona, College of Medicine||Program in Immunology, Clinical Research Division, and Immunotherapy Integrated Research Center, Fred Hutchinson Cancer Research CenterDepartment of Immunobiology, University of Arizona College of Medicine||University of Arizona Center on Aging, University of Arizona, College of MedicineDepartment of Immunobiology, University of Arizona College of Medicine||University of Arizona Center on Aging, University of Arizona, College of MedicineProgram in Immunology, Clinical Research Division, and Immunotherapy Integrated Research Center, Fred Hutchinson Cancer Research Center||Department of Immunology, University of WashingtonDepartment of Immunobiology, University of Arizona College of Medicine||University of Arizona Center on Aging, University of Arizona, College of MedicineBlood Systems Research Institute, Vitalant Research InstituteDepartment of Pediatrics, Section of Pediatric Tropical Medicine and National School of Tropical Medicine, Baylor College of Medicine||William T. Shearer Center for Human Immunobiology, Texas Children?ˉs HospitalDepartment of Medicine and Immunology Program, Memorial Sloan Kettering Cancer Center||Immunology Program, Memorial Sloan Kettering Cancer CenterDepartment of Immunobiology, University of Arizona College of Medicine||University of Arizona Center on Aging, University of Arizona, College of MedicineBlood Systems Research Institute, Vitalant Research InstituteDepartment of Immunobiology, University of Arizona College of Medicine||University of Arizona Center on Aging, University of Arizona, College of Medicine
基础医学
Nikolich-?ugich Janko,Bradshaw Christine M.,Coplen Christopher P.,Dudakov Jarrod,Uhrlaub Jennifer L.,Lanteri Marion C.,Murray Kristy O.,van den Brink Marcel R. M.,Jergovi? Mladen,Busch Michael P.,Sonar Sandip.Quantitative Restoration of Immune Defense in Old Animals Determined by Na?ve Antigen-Specific CD8 T cell Numbers[EB/OL].(2025-03-28)[2025-05-15].https://www.biorxiv.org/content/10.1101/2021.08.09.453882.点此复制
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