Loss of transcriptional heterogeneity in aged human muscle stem cells
Loss of transcriptional heterogeneity in aged human muscle stem cells
ABSTRACT Age-related loss of muscle mass and function negatively impacts healthspan and lifespan. Satellite cells function as muscle stem cells in muscle maintenance and regeneration by self-renewal, activation, proliferation and differentiation. These processes are perturbed in aging at the stem cell population level, contributing to muscle loss. However, how representation of subpopulations within the human satellite cell pool change during aging remains poorly understood. We previously reported a comprehensive baseline of human satellite cell (Hu-MuSCs) transcriptional activity in muscle homeostasis describing functional heterogenous human satellite cell subpopulations such as CAV1+ Hu-MUSCs (Barruet et al., 2020). Here, we sequenced additional satellite cells from new healthy donors and performed extended transcriptomic analyses with regard to aging. We found an age-related loss of global transcriptomic heterogeneity and identified new markers (CAV1, CXCL14, GPX3) along with previously described ones (FN1, ITGB1, SPRY1) that are altered during aging in human satellite cells. These findings describe new transcriptomic changes that occur during aging in human satellite cells and provide a foundation for understanding functional impact.
Marangoni Pauline、Pomerantz Jason H.、Barruet Emilie、Striedinger Katharine
Program in Craniofacial Biology and Department of Orofacial Sciences, University of CaliforniaDepartments of Surgery and Orofacial Sciences, Division of Plastic and Reconstructive Surgery, Program in Craniofacial Biology, Eli and Edythe Broad Center of Regeneration Medicine, University of California San FranciscoDepartments of Surgery and Orofacial Sciences, Division of Plastic and Reconstructive Surgery, Program in Craniofacial Biology, Eli and Edythe Broad Center of Regeneration Medicine, University of California San Francisco||Program in Craniofacial Biology and Department of Orofacial Sciences, University of CaliforniaDepartments of Surgery and Orofacial Sciences, Division of Plastic and Reconstructive Surgery, Program in Craniofacial Biology, Eli and Edythe Broad Center of Regeneration Medicine, University of California San Francisco||Program in Craniofacial Biology and Department of Orofacial Sciences, University of California
基础医学细胞生物学分子生物学
Marangoni Pauline,Pomerantz Jason H.,Barruet Emilie,Striedinger Katharine.Loss of transcriptional heterogeneity in aged human muscle stem cells[EB/OL].(2025-03-28)[2025-06-18].https://www.biorxiv.org/content/10.1101/2022.05.11.491465.点此复制
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