A comprehensive catalog with 100 million genes and 3,000 metagenome-assembled genomes from global cold seep sediments
A comprehensive catalog with 100 million genes and 3,000 metagenome-assembled genomes from global cold seep sediments
Abstract Cold seeps harbor abundant and diverse microbes that represent a tremendous potential for biological applications and also have a significant influence on biogeochemical cycles. Though recent metagenomic studies have expanded our understanding of the microbial community and function of seep microorganisms, the knowledge of diversity and genetic repertoire of global seep microbes is lacking. Here, we collected a compilation of 165 metagenomic data from 16 cold seep sites across the globe to construct comprehensive gene and genome catalogs. The non-redundant gene catalog was comprised of 147 million genes (clustered at 95% amino acid identity), and 35.72% of them could not be assigned to a function with the currently available databases. A total of 3,164 species-level representative metagenome-assembled genomes (MAGs) are obtained, most of which (94.31%) belong to novel species. Of them, 81 ANME species are identified covering all subclades except ANME-2d, and 23 syntrophic SRB species spanning Seep-SRB1a Seep-SRB1g, and Seep-SRB2 clades. The non-redundant gene and MAGs catalogs are a valuable resource that enables expanded knowledge of the structure and functions of cold seep microbiomes.
Zhao Zhuoming、Peng Yongyi、Shao Zongze、Zhang Chuwen、Liao Jing、Dong Xiyang、Jiang Qiuyun、Liu Qing、Han Yingchun
Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural ResourcesKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources||School of Marine Sciences, Sun Yat-Sen UniversityKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural ResourcesKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural ResourcesKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural ResourcesKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural ResourcesKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural ResourcesSchool of Marine Sciences, Sun Yat-Sen UniversityKey Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources
环境科学理论环境科学技术现状微生物学
Zhao Zhuoming,Peng Yongyi,Shao Zongze,Zhang Chuwen,Liao Jing,Dong Xiyang,Jiang Qiuyun,Liu Qing,Han Yingchun.A comprehensive catalog with 100 million genes and 3,000 metagenome-assembled genomes from global cold seep sediments[EB/OL].(2025-03-28)[2025-06-19].https://www.biorxiv.org/content/10.1101/2023.04.10.536201.点此复制
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