Testing theoretical minimal genomes using whole-cell models
Testing theoretical minimal genomes using whole-cell models
Abstract The minimal gene set for life has often been theorised, with at least ten produced for Mycoplasma genitalium (M. genitalium). Due to the difficulty of using M. genitalium in the lab, combined with its long replication time of 12 - 15 hours, none of these theoretical minimal genomes have been tested, even with modern techniques. The publication of the M. genitalium whole-cell model provided the first opportunity to test them, simulating the genome edits in-silico. We simulated eight minimal gene sets from the literature, finding that they produced in-silico cells that did not divide. Using knowledge from previous research, we reintroduced specific essential and low essential genes in-silico; enabling cellular division. This reinforces the need to identify species-specific low essential genes and their interactions. Any genome designs created using the currently incomplete and fragmented gene essentiality information, will very likely require in-vivo reintroductions to correct issues and produce dividing cells.
Chalkley Oliver、Marucci Lucia、Grierson Claire、Rees-Garbutt Joshua、Rightmyer Jake
BrisSynBio, University of Bristol||Bristol Centre for Complexity Sciences, Department of Engineering Mathematics, University of Bristol||Department of Engineering Mathematics, University of BristolBrisSynBio, University of Bristol||Department of Engineering Mathematics, University of Bristol||School of Cellular and Molecular Medicine, University of BristolBrisSynBio, University of Bristol||School of Biological Sciences, University of Bristol, Bristol Life Sciences BuildingBrisSynBio, University of Bristol||School of Biological Sciences, University of Bristol, Bristol Life Sciences BuildingSchool of Biological Sciences, University of Bristol, Bristol Life Sciences Building
生物科学研究方法、生物科学研究技术遗传学微生物学
Chalkley Oliver,Marucci Lucia,Grierson Claire,Rees-Garbutt Joshua,Rightmyer Jake.Testing theoretical minimal genomes using whole-cell models[EB/OL].(2025-03-28)[2025-06-15].https://www.biorxiv.org/content/10.1101/2020.03.26.010363.点此复制
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