Capturing volumetric dynamics at high speed in the brain by confocal light field microscopy
Capturing volumetric dynamics at high speed in the brain by confocal light field microscopy
Abstract Neural network performs complex computations through coordinating collective neural dynamics that are fast and in three-dimensions. Meanwhile, its proper function relies on its 3D supporting environment, including the highly dynamic vascular system that drives energy and material flow. Better understanding of these processes requires methods to capture fast volumetric dynamics in thick tissue. This becomes challenging due to the trade-off between speed and optical sectioning capability in conventional imaging techniques. Here we present a new imaging method, confocal light field microscopy, to enable fast volumetric imaging deep into brain. We demonstrated the power of this method by recording whole brain calcium transients in freely swimming larval zebrafish and observed behaviorally correlated activities on single neurons during its prey capture. Furthermore, we captured neural activities and circulating blood cells over a volume ? 800 μm × 150 μm at 70 Hz and up to 600 μm deep in the mice brain.
Bai Lu、Cong Lin、Du Jiulin、Yu Peng、Shi Wanzhuo、Li Funing、Wang Kai、Zhang Zhenkun、Zhang Tianlei
Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences||University of Chinese Academy of SciencesInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of SciencesInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences||University of Chinese Academy of Sciences||Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai 201210Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences||University of Chinese Academy of SciencesSchool of Physical Science, University of Science and Technology of ChinaInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences||University of Chinese Academy of SciencesInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences||University of Chinese Academy of Sciences||Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai 201210Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences||University of Chinese Academy of SciencesInstitute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences||University of Chinese Academy of Sciences
生物科学研究方法、生物科学研究技术生物物理学
Bai Lu,Cong Lin,Du Jiulin,Yu Peng,Shi Wanzhuo,Li Funing,Wang Kai,Zhang Zhenkun,Zhang Tianlei.Capturing volumetric dynamics at high speed in the brain by confocal light field microscopy[EB/OL].(2025-03-28)[2025-05-04].https://www.biorxiv.org/content/10.1101/2020.01.04.890624.点此复制
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