Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021
ABSTRACT BackgroundSince its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2, become dominant in several European countries raising great concern. AimThe aim of this study was to develop a duplex real-time RT-qPCR assay to detect, discriminate and quantitate SARS-CoV-2 variants containing one of its mutation signatures, the ΔHV69/70 deletion, to trace the community circulation of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). ResultsB.1.1.7 variant was first detected in sewage from the Southern city of Málaga (Andalucía) in week 20_52, and multiple introductions during Christmas holidays were inferred in different parts of the country, earlier than clinical epidemiological reporting by the local authorities. Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and provided information at the local level. Data from WWTPs which reached B.1.1.7 prevalences higher than 90% for ≥ 2 consecutive weeks showed that 8.1±1.8 weeks were required for B.1.1.7 to become dominant. ConclusionThe study highlights the applicability of RT-qPCR-based strategies to track specific mutations of variants of concern (VOCs) as soon as they are identified by clinical sequencing, and its integration into existing wastewater surveillance programs, as a cost-effective approach to complement clinical testing during the COVID-19 pandemic.
Carcereny Albert、Palau Margarita、Quer Josep、Mart¨anez-Vel¨￠zquez Ad¨￠n、Bosch Albert、Allende Ana、Gregori Josep、Guix Susana、Lois Marta、Cascales Jenifer、Romalde Jes¨2s L、D¨aaz-Reolid Azahara、Polo David、Ant¨?n Andr¨|s、Pint¨? Rosa M、Truchado Pilar、P¨|rez-Catalu?a Alba、Gonz¨￠lez Ruano Cristina、S¨￠nchez Gloria、Garcia-Cehic Damir
Enteric Virus laboratory, Department of Genetics, Microbiology and Statistics, Section of Microbiology, Virology and Biotechnology, School of Biology, and Institute of Nutrition and Food Safety (INSA), University of BarcelonaGeneral Directorate of Public Health, Ministry of HealthLiver Unit, Liver Diseases - Viral Hepatitis, Vall d?ˉHebron Institut de Recerca (VHIR), Vall d?ˉHebron Barcelona Hospital Campus||Centro de Investigaci¨?n Biom¨|dica en Red de Enfermedades Hep¨￠ticas y Digestivas (CIBERehd), Instituto de Salud Carlos IIIEnteric Virus laboratory, Department of Genetics, Microbiology and Statistics, Section of Microbiology, Virology and Biotechnology, School of Biology, and Institute of Nutrition and Food Safety (INSA), University of BarcelonaEnteric Virus laboratory, Department of Genetics, Microbiology and Statistics, Section of Microbiology, Virology and Biotechnology, School of Biology, and Institute of Nutrition and Food Safety (INSA), University of BarcelonaResearch Group on Microbiology and Quality of Fruit and Vegetables, CEBAS-CSIC, Campus Universitario de EspinardoLiver Unit, Liver Diseases - Viral Hepatitis, Vall d?ˉHebron Institut de Recerca (VHIR), Vall d?ˉHebron Barcelona Hospital Campus||Centro de Investigaci¨?n Biom¨|dica en Red de Enfermedades Hep¨￠ticas y Digestivas (CIBERehd), Instituto de Salud Carlos IIIEnteric Virus laboratory, Department of Genetics, Microbiology and Statistics, Section of Microbiology, Virology and Biotechnology, School of Biology, and Institute of Nutrition and Food Safety (INSA), University of BarcelonaDepartment of Microbiology and Parasitology, CIBUS-Faculty of Biology & Institute CRETUS, Universidade de Santiago de CompostelaResearch Group on Microbiology and Quality of Fruit and Vegetables, CEBAS-CSIC, Campus Universitario de EspinardoDepartment of Microbiology and Parasitology, CIBUS-Faculty of Biology & Institute CRETUS, Universidade de Santiago de CompostelaDepartment of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food TechnologyDepartment of Microbiology and Parasitology, CIBUS-Faculty of Biology & Institute CRETUS, Universidade de Santiago de CompostelaMicrobiology Department, Vall d?ˉHebron Institut de Recerca (VHIR), Vall d?ˉHebron Barcelona Hospital CampusEnteric Virus laboratory, Department of Genetics, Microbiology and Statistics, Section of Microbiology, Virology and Biotechnology, School of Biology, and Institute of Nutrition and Food Safety (INSA), University of BarcelonaResearch Group on Microbiology and Quality of Fruit and Vegetables, CEBAS-CSIC, Campus Universitario de EspinardoDepartment of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food TechnologySubdirecci¨?n General de Protecci¨?n de las Aguas y Gesti¨?n de Riesgos, Ministerio para la Transici¨?n Ecol¨?gica y el Reto Demogr¨￠ficoDepartment of Preservation and Food Safety Technologies, Institute of Agrochemistry and Food TechnologyLiver Unit, Liver Diseases - Viral Hepatitis, Vall d?ˉHebron Institut de Recerca (VHIR), Vall d?ˉHebron Barcelona Hospital Campus||Centro de Investigaci¨?n Biom¨|dica en Red de Enfermedades Hep¨￠ticas y Digestivas (CIBERehd), Instituto de Salud Carlos III
医学研究方法预防医学环境生物学
Carcereny Albert,Palau Margarita,Quer Josep,Mart¨anez-Vel¨￠zquez Ad¨￠n,Bosch Albert,Allende Ana,Gregori Josep,Guix Susana,Lois Marta,Cascales Jenifer,Romalde Jes¨2s L,D¨aaz-Reolid Azahara,Polo David,Ant¨?n Andr¨|s,Pint¨? Rosa M,Truchado Pilar,P¨|rez-Catalu?a Alba,Gonz¨￠lez Ruano Cristina,S¨￠nchez Gloria,Garcia-Cehic Damir.Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021[EB/OL].(2025-03-28)[2025-04-28].https://www.medrxiv.org/content/10.1101/2021.05.27.21257918.点此复制
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