The bacterial leader peptide peTrpL has a conserved function in antibiotic-dependent posttranscriptional regulation of ribosomal genes
The bacterial leader peptide peTrpL has a conserved function in antibiotic-dependent posttranscriptional regulation of ribosomal genes
Summary The ribosome-dependent attenuator located upstream of bacterial tryptophan biosynthesis genes harbors a small ORF trpL containing tryptophan codons. When tryptophan is available, efficient trpL translation causes transcription termination and release of the attenuator RNA rnTrpL. In Sinorhizobium meliloti, rnTrpL is a trans-acting sRNA. Here, we identified an evolutionary conserved function for the trpL-encoded 14-aa leader peptide peTrpL. Upon exposure to tetracycline, the cellular peTrpL levels were increased and rnTrpL was generated independently of tryptophan availability. Both peTrpL and rnTrpL were found to be involved in tetracycline-dependent destabilization of rplUrpmA mRNA encoding ribosomal proteins L21 and L27. We provide evidence for redirection of the sRNA rnTrpL from its antibiotic-independent target trpDC to rplUrpmA by formation of an antibiotic-dependent ribonucleoprotein complex (ARNP). ARNPs comprising peTrpL, rnTrpL, rplUrpmA and antisense RNA were also observed for other translation-inhibiting antibiotics, suggesting that bacteria evolved mechanisms to utilize antibiotics for mRNA destabilization.
Barth-Weber Susanne、Baumgardt Kathrin、Chervontseva Zoe、Melior Hendrik、Maa? Sandra、Li Siqi、F?rstner Konrad U.、Ziebuhr John、Ahrens Christian H.、St?tzel Maximilian、Evguenieva-Hackenberg Elena、Becher D?rte、Varadarajan Adithi R.、Sch¨1tz Rubina、Shevkoplias Aleksei、Azarderakhsh Saina
Institute of Microbiology and Molecular Biology, University of GiessenInstitute of Microbiology and Molecular Biology, University of GiessenCenter for Life Sciences, Skolkovo Institute of Technology||Kharkevich Institute for Information Transmission Problems RASInstitute of Microbiology and Molecular Biology, University of GiessenInstitute of Microbiology, University of GreifswaldInstitute of Microbiology and Molecular Biology, University of GiessenZB MED - Information Center for Life Sciences, University of CologneInstitute of Medical Virology, University of GiessenAgroscope & SIB Swiss Institute of BioinformaticsInstitute of Microbiology and Molecular Biology, University of GiessenInstitute of Microbiology and Molecular Biology, University of GiessenInstitute of Microbiology, University of GreifswaldAgroscope & SIB Swiss Institute of BioinformaticsInstitute of Microbiology and Molecular Biology, University of GiessenLaboratory for Marine Benthic Ecology and Hydrobiology||Faculty of Biology and Biotechnology, Higher School of EconomicsInstitute of Microbiology and Molecular Biology, University of Giessen
分子生物学微生物学生物化学
Barth-Weber Susanne,Baumgardt Kathrin,Chervontseva Zoe,Melior Hendrik,Maa? Sandra,Li Siqi,F?rstner Konrad U.,Ziebuhr John,Ahrens Christian H.,St?tzel Maximilian,Evguenieva-Hackenberg Elena,Becher D?rte,Varadarajan Adithi R.,Sch¨1tz Rubina,Shevkoplias Aleksei,Azarderakhsh Saina.The bacterial leader peptide peTrpL has a conserved function in antibiotic-dependent posttranscriptional regulation of ribosomal genes[EB/OL].(2025-03-28)[2025-05-21].https://www.biorxiv.org/content/10.1101/606483.点此复制
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