DNA-dependent RNA cleavage by the Natronobacterium gregoryi Argonaute
DNA-dependent RNA cleavage by the Natronobacterium gregoryi Argonaute
Abstract We show here that, unlike most other prokaryotic Argonaute (Ago) proteins, which are DNA-guided endonucleases, the Natronobacterium gregoryi-derived Ago (NgAgo) can function as a DNA-guided endoribonuclease, cleaving RNA, rather than DNA, in a targeted manner. The NgAgo protein, in complex with 5’-hydroxylated or 5’-phosphrylated oligodeoxyribonucleotides (ODNs) of variable lengths, split RNA targets into two or more fragments in vitro, suggesting its physiological role in bacteria and demonstrating a potential for degrading RNA molecules such as mRNA or lncRNA in eukaryotic cells in a targeted manner.
Kim Yoon Young、Lee Kang-In、Kim Seokjoong、Habib Omer、Kim Jin-Soo、Ye Sunghyeok、Bae Taegeun、Kim Kyoungmi、Lee Seung Hwan
ToolGen, IncToolGen, IncToolGen, IncCenter for Genome Engineering, Institute for Basic ScienceCenter for Genome Engineering, Institute for Basic Science||IBS School, University of Science and Technology||Department of Chemistry, Seoul National UniversityCenter for Genome Engineering, Institute for Basic Science||IBS School, University of Science and TechnologyCenter for Genome Engineering, Institute for Basic Science||IBS School, University of Science and TechnologyCenter for Genome Engineering, Institute for Basic ScienceCenter for Genome Engineering, Institute for Basic Science
分子生物学遗传学
Kim Yoon Young,Lee Kang-In,Kim Seokjoong,Habib Omer,Kim Jin-Soo,Ye Sunghyeok,Bae Taegeun,Kim Kyoungmi,Lee Seung Hwan.DNA-dependent RNA cleavage by the Natronobacterium gregoryi Argonaute[EB/OL].(2025-03-28)[2025-06-28].https://www.biorxiv.org/content/10.1101/101923.点此复制
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