A combined pipeline for quantitative analysis of human brain cytoarchitecture
A combined pipeline for quantitative analysis of human brain cytoarchitecture
Abstract The 3D analysis of the human brain architecture at cellular resolution is still a big challenge. In this work, we propose a pipeline that solves the problem of performing neuronal mapping in large human brain samples at micrometer resolution. First, we introduce the SWITCH/TDE protocol: a robust methodology to clear and label human brain tissue. Then, we implement the 2.5D method based on a Convolutional Neural Network, to automatically detect and segment all neurons. Our method proved to be highly versatile and was applied successfully on specimens from different areas of the cortex originating from different subjects (young, adult and elderly, both healthy and pathological). We quantitatively evaluate the density and, more importantly, the mean volume of the thousands of neurons identified within the specimens. In conclusion, our pipeline makes it possible to study the structural organization of the brain and expands the histopathological studies to the third dimension.
Mazzamuto Giacomo、Roffilli Matteo、Neri Mattia、Lughi Giovanni、Pesce Luca、Destrieux Christophe、Castelli Filippo、Lazzeri Erica、Pavone Francesco S.、Costantini Irene、Conti Valerio、Laurino Annunziatina、Guerrini Renzo、Simonetto Andrea、Silvestri Ludovico
National Institute of Optics (INO), National Research Council (CNR)||European Laboratory for Non-Linear Spectroscopy (LENS), University of FlorenceBioretics srlBioretics srlBioretics srlEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence||Department of Physics, University of FlorenceUMR 1253, iBrain, Universit¨| de Tours, InsermEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence||Department of Physics, University of FlorenceEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence||Department of Physics, University of FlorenceEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence||National Institute of Optics (INO), National Research Council (CNR)||Department of Physics, University of FlorenceEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence||National Institute of Optics (INO), National Research Council (CNR)Pediatric Neurology, Neurogenetics and Neurobiology Unit and Laboratories, A. Meyer Children?ˉs Hospital, University of FlorenceEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of FlorencePediatric Neurology, Neurogenetics and Neurobiology Unit and Laboratories, A. Meyer Children?ˉs Hospital, University of FlorenceBioretics srlEuropean Laboratory for Non-Linear Spectroscopy (LENS), University of Florence||National Institute of Optics (INO), National Research Council (CNR)||Department of Physics, University of Florence
基础医学生物科学研究方法、生物科学研究技术神经病学、精神病学
Mazzamuto Giacomo,Roffilli Matteo,Neri Mattia,Lughi Giovanni,Pesce Luca,Destrieux Christophe,Castelli Filippo,Lazzeri Erica,Pavone Francesco S.,Costantini Irene,Conti Valerio,Laurino Annunziatina,Guerrini Renzo,Simonetto Andrea,Silvestri Ludovico.A combined pipeline for quantitative analysis of human brain cytoarchitecture[EB/OL].(2025-03-28)[2025-04-24].https://www.biorxiv.org/content/10.1101/2020.08.06.219444.点此复制
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