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Volume regulation in adhered cells: roles of surface tension and cell swelling

Volume regulation in adhered cells: roles of surface tension and cell swelling

来源:bioRxiv_logobioRxiv
英文摘要

The volume of adhered cells has been shown experimentally to decrease during spreading. This effect can be understood from the pump-leak model, which we have extended to include mechano-sensitive ion transporters. We identify a novel effect that has important consequences on cellular volume loss; cells that are swollen due to a modulation of ion transport rates are more susceptible to volume loss in response to a tension increase. This effect explains in a plausible manner the discrepancies between three recent, independent experiments on adhered cells, between which both the magnitude of the volume change and its dynamics varied substantially. We suggest that starved and synchronized cells in two of the experiments were in a swollen state and, consequently, exhibited a large volume loss at steady state. Non-swollen cells, for which there is a very small steady-state volume decrease, are still predicted to transiently lose volume during spreading due to a relaxing viscoelastic tension that is large compared with the steady-state tension. We elucidate the roles of cell swelling and surface tension in cellular volume regulation and discuss their possible microscopic origins.

Safran Samuel A、Joanny Jean-Francois、Sens Pierre、Adar Ram M、Singh Vishen Amit

10.1101/2022.08.24.505072

细胞生物学生物物理学生理学

Safran Samuel A,Joanny Jean-Francois,Sens Pierre,Adar Ram M,Singh Vishen Amit.Volume regulation in adhered cells: roles of surface tension and cell swelling[EB/OL].(2025-03-28)[2025-05-07].https://www.biorxiv.org/content/10.1101/2022.08.24.505072.点此复制

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