Exercise promotes satellite cell contribution to myofibers in a load-dependent manner
Exercise promotes satellite cell contribution to myofibers in a load-dependent manner
Abstract Satellite cells (SCs) are required for muscle repair following injury and are involved in muscle remodeling upon muscular contractions. Exercise stimulates SC accumulation and myonuclear accretion. To what extent exercise training at different mechanical loads drive SC contribution to myonuclei however is unknown. By performing SC fate tracing experiments, we show that 8-weeks of voluntary wheel running increased SC contribution to myonuclei in mouse plantar flexor muscles in a load-dependent but fiber type-independent manner. Increased SC fusion however was not exclusively linked to muscle hypertrophy as wheel running without external load substantially increased SC fusion in the absence of fiber hypertrophy. Due to nuclear propagation, nuclear fluorescent fate tracing mouse models were inadequate to quantify SC contribution to myonuclei. Ultimately, by performing fate tracing at the DNA level, we show that SC contribution mirrors myonuclear accretion during exercise. Collectively, these findings provide direct evidence that mechanical load during exercise independently promotes SC contribution to existing myofibers.
Masschelein Evi、De Bock Katrien、D?ˉHulst Gommaar、Hinte Laura、Soro-Arnaiz In¨|s、Gorski Tatiane、Zvick Joel、Bar-Nur Ori、von Meyenn Ferdinand
Department Health Sciences and Technology, Laboratory of Exercise and Health, Swiss Federal Institute of Technology (ETH) ZurichDepartment Health Sciences and Technology, Laboratory of Exercise and Health, Swiss Federal Institute of Technology (ETH) ZurichDepartment Health Sciences and Technology, Laboratory of Exercise and Health, Swiss Federal Institute of Technology (ETH) ZurichDepartment Health Sciences and Technology, Laboratory of Nutrition and Metabolic Epigenetics, Swiss Federal Institute of Technology (ETH) ZurichDepartment Health Sciences and Technology, Laboratory of Exercise and Health, Swiss Federal Institute of Technology (ETH) ZurichDepartment Health Sciences and Technology, Laboratory of Exercise and Health, Swiss Federal Institute of Technology (ETH) ZurichDepartment Health Sciences and Technology, Laboratory of Regenerative and Movement Biology, Swiss Federal Institute of Technology (ETH) ZurichDepartment Health Sciences and Technology, Laboratory of Regenerative and Movement Biology, Swiss Federal Institute of Technology (ETH) ZurichDepartment Health Sciences and Technology, Laboratory of Nutrition and Metabolic Epigenetics, Swiss Federal Institute of Technology (ETH) Zurich
基础医学细胞生物学生理学
Masschelein Evi,De Bock Katrien,D?ˉHulst Gommaar,Hinte Laura,Soro-Arnaiz In¨|s,Gorski Tatiane,Zvick Joel,Bar-Nur Ori,von Meyenn Ferdinand.Exercise promotes satellite cell contribution to myofibers in a load-dependent manner[EB/OL].(2025-03-28)[2025-05-24].https://www.biorxiv.org/content/10.1101/2020.03.19.998211.点此复制
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