Site-specific Halogenation of Peptides and Proteins using engineered Halogenase Enzymes
Site-specific Halogenation of Peptides and Proteins using engineered Halogenase Enzymes
Abstract We demonstrate novel in vitro halogenation of peptides by halogenase enzymes, and identify the (G/S)GW motif (HaloTryp Tag) as a preferred substrate. We further derive PyrH halogenase mutants showing improved halogenation of the HaloTryp Tag, both as a free peptide and when genetically fused to model proteins.
Seayad Jayasree、Sana Barindra、Ho Timothy、Ke Ding、Yen Li Eunice Hui、Ghadessy Farid J、Duong Hung A
Institute of Chemical And Engineering SciencesDisease Intervention Technology Laboratory, Institute of Molecular and Cellular BiologyInstitute of Chemical And Engineering SciencesDisease Intervention Technology Laboratory, Institute of Molecular and Cellular BiologyInstitute of Chemical And Engineering SciencesInstitute of Chemical And Engineering Sciences
生物科学研究方法、生物科学研究技术生物化学分子生物学
Seayad Jayasree,Sana Barindra,Ho Timothy,Ke Ding,Yen Li Eunice Hui,Ghadessy Farid J,Duong Hung A.Site-specific Halogenation of Peptides and Proteins using engineered Halogenase Enzymes[EB/OL].(2025-03-28)[2025-07-01].https://www.biorxiv.org/content/10.1101/2022.07.19.500721.点此复制
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