A pervasive large conjugative plasmid mediates multispecies biofilm formation in the intestinal microbiota increasing resilience to perturbations
A pervasive large conjugative plasmid mediates multispecies biofilm formation in the intestinal microbiota increasing resilience to perturbations
SUMMARY Although horizontal gene transfer is pervasive in the intestinal microbiota, we understand only superficially the roles of most exchanged genes and how the mobile repertoire affects community dynamics. Similarly, little is known about the mechanisms underlying the ability of a community to recover after a perturbation. Here, we identified and functionally characterized a large conjugative plasmid that is one of the most frequently transferred elements among Bacteroidales species and is ubiquitous in diverse human populations. This plasmid encodes both an extracellular polysaccharide and fimbriae, which promote the formation of multispecies biofilms in the mammalian gut. We use a hybridization-based approach to visualize biofilms in clarified whole colon tissue with unprecedented 3D spatial resolution. These biofilms increase bacterial survival to common stressors encountered in the gut, increasing strain resiliency, and providing a rationale for the plasmid’s recent spread and high worldwide prevalence.
Said Nathania、Camacho Adriana Gutierrez、Kov¨￠cs ¨￠kos T.、Waldor Matthew K.、Yildiz Fitnat H.、Hutt Kathryn、Sheahan Madeline L.、Elmekki Nazik M.、Coyne Michael J.、Comstock Laurie E.、Flores Katia、Garc¨aa-Bayona Leonor
Duchossois Family Institute, University of Chicago||Department of Microbiology, University of ChicagoPonce Health Sciences UniversityInstitute of Biology Leiden, Leiden UniversityDivision of Infectious Diseases, Brigham & Women?ˉs Hospital||Department of Microbiology, Harvard Medical School||Howard Hughes Medical InstituteDepartment of Microbiology and Environmental Toxicology, University of CaliforniaAnimal Resources Center, University of ChicagoDuchossois Family Institute, University of Chicago||Department of Microbiology, University of ChicagoDuchossois Family Institute, University of Chicago||Department of Microbiology, University of ChicagoDuchossois Family Institute, University of Chicago||Department of Microbiology, University of Chicago||Division of Infectious Diseases, Brigham & Women?ˉs HospitalDuchossois Family Institute, University of Chicago||Department of Microbiology, University of Chicago||Division of Infectious Diseases, Brigham & Women?ˉs HospitalDuchossois Family Institute, University of Chicago||Department of Microbiology, University of ChicagoDuchossois Family Institute, University of Chicago||Department of Microbiology, University of Chicago||Division of Infectious Diseases, Brigham & Women?ˉs Hospital
微生物学分子生物学环境生物学
Said Nathania,Camacho Adriana Gutierrez,Kov¨￠cs ¨￠kos T.,Waldor Matthew K.,Yildiz Fitnat H.,Hutt Kathryn,Sheahan Madeline L.,Elmekki Nazik M.,Coyne Michael J.,Comstock Laurie E.,Flores Katia,Garc¨aa-Bayona Leonor.A pervasive large conjugative plasmid mediates multispecies biofilm formation in the intestinal microbiota increasing resilience to perturbations[EB/OL].(2025-03-28)[2025-05-21].https://www.biorxiv.org/content/10.1101/2024.04.29.590671.点此复制
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