Repeated gain and loss of a single gene modulates the evolution of vascular pathogen lifestyles
Repeated gain and loss of a single gene modulates the evolution of vascular pathogen lifestyles
Abstract Vascular pathogens travel long distances through host veins leading to life-threatening, systemic infections. In contrast, non-vascular pathogens remain restricted to infection sites, triggering localized symptom development. The contrasting features of vascular and non-vascular diseases suggest distinct etiologies, but the basis for each remains unclear. Here, we show that the hydrolase CbsA acts as a phenotypic switch between vascular and non-vascular plant pathogenesis. cbsA was enriched in genomes of vascular phytopathogenic bacteria in the Xanthomonadaceae family and absent in most non-vascular species. CbsA expression allowed non-vascular Xanthomonas to cause vascular blight while cbsA mutagenesis resulted in reduction of vascular or enhanced non-vascular symptom development. Phylogenetic hypothesis testing further revealed that cbsA was lost in multiple non-vascular lineages and more recently gained by some vascular subgroups, suggesting that vascular pathogenesis is ancestral. Our results overall demonstrate how the gain and loss of single loci can facilitate the evolution of complex ecological traits.
Vancheva Taca、Lang Jillian M.、Gagnevin Lionel、Sonti Ramesh V、de la Fuente Leonardo、Bragard Claude、Gluck-Thaler Emile、Cerutti Aude、Perez-Quintero Alvaro、Butchacas Jules、Roman-Reyna Ver¨?nica、Madhaven Vishnu Narayanan、Pesce C¨|line、Koebnik Ralf、Szurek Boris、Allen Caitilyn、Jacobs Jonathan M.、Slot Jason C.、Verdier Valerie、Merfa Marcus V.、Jauneau Alain、Patel Hitendra Kumar、Shantharaj Deepak、Cunnac S¨|bastien、Beckham Gregg、No?l Laurent D.、Leach Jan E.
Institut de Recherche pour le D¨|veloppement, UMR Interactions Plantes Microorganismes Environnement||Earth & Life Institute, Universit¨| Catholique Louvain-la-NeuveAgricultural Biology, Colorado State UniversityInstitut de Recherche pour le D¨|veloppement, UMR Interactions Plantes Microorganismes EnvironnementCSIR-Centre for Cellular and Molecular BiologyDepartment of Entomology and Plant Pathology, Auburn UniversityEarth & Life Institute, Universit¨| Catholique Louvain-la-NeuveDepartment of Plant Pathology, The Ohio State UniversityLIPM, Universit¨| de Toulouse, INRA, CNRS, Universit¨| Paul SabatierAgricultural Biology, Colorado State UniversityDepartment of Plant Pathology, The Ohio State University||Infectious Disease Institute, The Ohio State UniversityDepartment of Plant Pathology, The Ohio State University||Infectious Disease Institute, The Ohio State UniversityCSIR-Centre for Cellular and Molecular BiologyInstitut de Recherche pour le D¨|veloppement, UMR Interactions Plantes Microorganismes Environnement||Earth & Life Institute, Universit¨| Catholique Louvain-la-Neuve||Department of Microbiology, University of New HampshireInstitut de Recherche pour le D¨|veloppement, UMR Interactions Plantes Microorganismes EnvironnementInstitut de Recherche pour le D¨|veloppement, UMR Interactions Plantes Microorganismes EnvironnementDepartment of Plant Pathology, University of Wisconsin?aMadisonDepartment of Plant Pathology, The Ohio State University||Infectious Disease Institute, The Ohio State UniversityDepartment of Plant Pathology, The Ohio State University||Infectious Disease Institute, The Ohio State UniversityInstitut de Recherche pour le D¨|veloppement, UMR Interactions Plantes Microorganismes EnvironnementDepartment of Entomology and Plant Pathology, Auburn UniversityInstitut F¨|d¨|ratif de Recherche 3450, Plateforme Imagerie, P?le de Biotechnologie V¨|g¨|taleCSIR-Centre for Cellular and Molecular BiologyDepartment of Entomology and Plant Pathology, Auburn UniversityInstitut de Recherche pour le D¨|veloppement, UMR Interactions Plantes Microorganismes EnvironnementNational Bioenergy Center, National Renewable Energy LaboratoryLIPM, Universit¨| de Toulouse, INRA, CNRS, Universit¨| Paul SabatierAgricultural Biology, Colorado State University
微生物学植物学分子生物学
Vancheva Taca,Lang Jillian M.,Gagnevin Lionel,Sonti Ramesh V,de la Fuente Leonardo,Bragard Claude,Gluck-Thaler Emile,Cerutti Aude,Perez-Quintero Alvaro,Butchacas Jules,Roman-Reyna Ver¨?nica,Madhaven Vishnu Narayanan,Pesce C¨|line,Koebnik Ralf,Szurek Boris,Allen Caitilyn,Jacobs Jonathan M.,Slot Jason C.,Verdier Valerie,Merfa Marcus V.,Jauneau Alain,Patel Hitendra Kumar,Shantharaj Deepak,Cunnac S¨|bastien,Beckham Gregg,No?l Laurent D.,Leach Jan E..Repeated gain and loss of a single gene modulates the evolution of vascular pathogen lifestyles[EB/OL].(2025-03-28)[2025-06-06].https://www.biorxiv.org/content/10.1101/2020.04.24.058529.点此复制
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