Surface-driven RNA-refolding by the OB-fold proteins of the Trypanosoma brucei editosome
Surface-driven RNA-refolding by the OB-fold proteins of the Trypanosoma brucei editosome
SUMMARY RNA editing in African trypanosomes represents an RNA-processing reaction that generates functional mitochondrial transcripts from sequence-deficient pre-mRNAs. The reaction is catalyzed by a macromolecular protein complex known as the editosome. Editosomes have been demonstrated to execute RNA-chaperone activity to overcome the highly folded nature of pre-edited substrate mRNAs. The molecular basis of this activity is unknown. Here we test five OB-fold proteins of the editosome as potential candidates. We show that the different proteins interact by hetero-oligomerization and we demonstrate that all proteins execute RNA-chaperone activity. Activity differences correlate with the surface areas of the proteins and map predominantly to the intrinsically disordered subdomains of the polypeptides. To provide a structural context for our findings we present a coarse-grained model of the editosome. The model suggests that an inner core of catalytically active editosome components is separated from an outer shell of IDP-domains that act as RNA-remodeling sites.
Kasprzak Joanna M.、Bytner Patrycia、Bujnicki Janusz M.、G?ringer H. Ulrich、Voigt Christin、Dobrych?op Mateusz、Czerwoniec Anna、Kruse Elisabeth
Institute of Molecular Biology and Biotechnology, Adam Mickiewicz UniversityInstitute of Molecular Biology and Biotechnology, Adam Mickiewicz UniversityInstitute of Molecular Biology and Biotechnology, Adam Mickiewicz University||Laboratory of Bioinformatics and Protein Engineering, International Institute of Molecular and Cell BiologyMolecular Genetics, Darmstadt University of TechnologyMolecular Genetics, Darmstadt University of TechnologyInstitute of Molecular Biology and Biotechnology, Adam Mickiewicz UniversityInstitute of Molecular Biology and Biotechnology, Adam Mickiewicz UniversityMolecular Genetics, Darmstadt University of Technology
分子生物学生物化学生物物理学
Kasprzak Joanna M.,Bytner Patrycia,Bujnicki Janusz M.,G?ringer H. Ulrich,Voigt Christin,Dobrych?op Mateusz,Czerwoniec Anna,Kruse Elisabeth.Surface-driven RNA-refolding by the OB-fold proteins of the Trypanosoma brucei editosome[EB/OL].(2025-03-28)[2025-08-02].https://www.biorxiv.org/content/10.1101/099705.点此复制
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