A plasma membrane nanoplatform ensures signal specificity during osmotic signaling in plants
A plasma membrane nanoplatform ensures signal specificity during osmotic signaling in plants
ABSTRACT In the course of their growth and development plants have to constantly perceive and react to their environment. This is achieved in cells, by the coordination of complex combinatorial signaling networks. However, how signal integration and specificity are achieved in this context is unknown. With a focus on the hyperosmotic stimulus, we use live super-resolution light imaging methods to demonstrate that a Rho GTPase, Rho-of-Plant 6 (ROP6), forms stimuli-dependant nanodomains within the PM. These nanodomains are necessary and sufficient to transduce production of reactive oxygen species (ROS),that act as secondary messengers and trigger several plant adaptive responses to osmotic constraints. Furthermore, ROP6 activation triggers the nanoclustering of two NADPH oxidases that subsequently generates ROS. ROP6 nanoclustering is also needed for cell surface auxin signaling, but short-time auxin treatment does not induce ROS accumulation. We show that auxin-induced ROP6 nanodomains, unlike osmotically-driven ROP6 clusters, do not recruit the NADPH oxidase, RBOHD. Together, our results suggest that Rho GTPase nano-partitioning at the PM ensure signal specificity downstream of independent stimuli.
Martiniere A.、Smokvarska M.、Francis C.、Platre M.P.、Alcon C.、Nacry P.、Jaillais Y.、Bayle V.、Maurel C.、Nollmann M.、Fiche J.B.、Dumont X.
BPMP, Univ MontpellierBPMP, Univ MontpellierBPMP, Univ MontpellierLaboratoire Reproduction et D¨|veloppement des Plantes, Universit¨| Lyon, ¨|cole Normale Sup¨|rieure de Lyon, Universit¨| Claude Bernard Lyon 1, Centre National de la Recherche Scientifique, Institut National de la Recherche AgronomiqueBPMP, Univ MontpellierBPMP, Univ MontpellierLaboratoire Reproduction et D¨|veloppement des Plantes, Universit¨| Lyon, ¨|cole Normale Sup¨|rieure de Lyon, Universit¨| Claude Bernard Lyon 1, Centre National de la Recherche Scientifique, Institut National de la Recherche AgronomiqueLaboratoire Reproduction et D¨|veloppement des Plantes, Universit¨| Lyon, ¨|cole Normale Sup¨|rieure de Lyon, Universit¨| Claude Bernard Lyon 1, Centre National de la Recherche Scientifique, Institut National de la Recherche AgronomiqueBPMP, Univ MontpellierCentre de Biochimie Structurale, Centre National de la Recherche Scientifique Unit¨| Mixte de Recherche 5048, Institut National de la Sant¨| et de la Recherche M¨|dicale U1054, Universit¨| de MontpellierCentre de Biochimie Structurale, Centre National de la Recherche Scientifique Unit¨| Mixte de Recherche 5048, Institut National de la Sant¨| et de la Recherche M¨|dicale U1054, Universit¨| de MontpellierBPMP, Univ Montpellier
细胞生物学植物学分子生物学
Martiniere A.,Smokvarska M.,Francis C.,Platre M.P.,Alcon C.,Nacry P.,Jaillais Y.,Bayle V.,Maurel C.,Nollmann M.,Fiche J.B.,Dumont X..A plasma membrane nanoplatform ensures signal specificity during osmotic signaling in plants[EB/OL].(2025-03-28)[2025-05-19].https://www.biorxiv.org/content/10.1101/2020.06.14.145961.点此复制
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