CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization
CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization
Epigenetic modifications play an essential role in chromatin architecture and dynamics. The role of epigenetic modification in chromatin organization has been studied by Hi-C from population cells, but imaging techniques to study their correlation and regulation in single living cells are lacking. Here we develop a CRISPR-based EpiGo (Epigenetic perturbation induced Genome organization) system to track epigenetic modification-mediated relocation, interaction or reorganization of genomic regions in living cells. EpiGo-KRAB is sufficient to induce the relocation of genomic loci to HP1α condensates and trigger genomic interactions. EpiGo-KRAB also triggers the induction of H3K9me3 at large genomic regions, which decorate on the surface of HP1α condensates possibly driven by phase separation.
Zhang Shaojie、Xie Wei、Wang Yao、Pederson Thoru、Xiao Xiao、Yang Chen、Feng Ying、Ma Hanhui、Naseri Ardalan、Zheng Jing
Department of Computer Science, University of Central FloridaCenter for Stem Cell Biology and Regenerative Medicine, MOE Key Laboratory of Bioinformatics, School of Life Sciences, THU-PKU Center for Life Sciences, Tsinghua UniversityCenter for Stem Cell Biology and Regenerative Medicine, MOE Key Laboratory of Bioinformatics, School of Life Sciences, THU-PKU Center for Life Sciences, Tsinghua UniversityDepartment of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical SchoolSchool of Biotechnology, East China University of Science and TechnologySchool of Life Science and Technology, ShanghaiTech University||School of Pharmacy, East China University of Science and TechnologySchool of Life Science and Technology, ShanghaiTech University||School of Biotechnology, East China University of Science and TechnologySchool of Life Science and Technology, ShanghaiTech UniversityDepartment of Computer Science, University of Central FloridaSchool of Pharmacy, East China University of Science and Technology
遗传学分子生物学细胞生物学
Zhang Shaojie,Xie Wei,Wang Yao,Pederson Thoru,Xiao Xiao,Yang Chen,Feng Ying,Ma Hanhui,Naseri Ardalan,Zheng Jing.CRISPR-based Live Imaging of Epigenetic Modification-Mediated Genome Reorganization[EB/OL].(2025-03-28)[2025-05-28].https://www.biorxiv.org/content/10.1101/2020.02.18.954610.点此复制
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