Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization
Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization
Abstract In many physiological situations, BAR proteins interact with, and reshape, pre-existing curved membranes, contributing to essential cellular processes. However, the non-equilibrium and timedependent process of reshaping, and its dependence on initial membrane shape, remains largely unknown. Here we explain, both experimentally and through modelling, how a BAR protein dynamically interacts with mechanically bent lipid membranes. We capture protein binding to curved membranes, and characterize a variety of dynamical reshaping events depending on membrane shape and protein arrangement. The events can be generally understood by an isotropic-to-nematic phase transition, in which low curvature templates with isotropic protein orientation progress towards highly curved lipid tubes with nematic protein arrangement. Our findings also apply in cells, where mechanical stretch triggers BAR-protein-membrane interactions that enable potential mechanotransduction mechanisms. Our results characterize and broaden the reshaping processes of BAR proteins on mechanically constrained membranes, demonstrating the interplay between membrane mechanical stimuli and BAR protein response.
Tozzi Caterina、Quiroga Xarxa、Staykova Margarita、Zalvidea Dobryna、Walani Nikhil、Trepat Xavier、Arroyo Marino、Roca-Cusachs Pere、Le Roux Anabel-Lise
Universitat Polit¨¨cnica de Catalunya (UPC)Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST)Department of Physics, University of DurhamInstitute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST)Universitat Polit¨¨cnica de Catalunya (UPC)Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST)||Universitat de Barcelona||Instituci¨? Catalana de Recerca i Estudis Avan?ats (ICREA), Passeig de Llu¨as Companys||Centro de Investigaci¨?n Biom¨|dica en Red de C¨￠ncer (CIBERONC)Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST)||Universitat Polit¨¨cnica de Catalunya (UPC)||Centre International de M¨¨todes Num¨¨rics en Enginyeria (CIMNE)Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST)||Universitat de BarcelonaInstitute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST)
生物科学理论、生物科学方法细胞生物学生物物理学
Tozzi Caterina,Quiroga Xarxa,Staykova Margarita,Zalvidea Dobryna,Walani Nikhil,Trepat Xavier,Arroyo Marino,Roca-Cusachs Pere,Le Roux Anabel-Lise.Dynamic Mechanochemical feedback between curved membranes and BAR protein self-organization[EB/OL].(2025-03-28)[2025-06-09].https://www.biorxiv.org/content/10.1101/2020.09.23.310169.点此复制
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