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ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater

ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater

来源:medRxiv_logomedRxiv
英文摘要

Abstract Wastewater was screened for the presence of functionally significant mutations in SARS-CoV-2 associated with emerging variants of concern (VOC) by ddPCR, and results accorded with sequencing of clinical samples from the same region. We propose that PCR-based screening of wastewater can provide a powerful tool for rapid and inexpensive screening of large population segments for VOC-associated mutations and can hone complementary sampling and sequencing of direct (human) test material to track emerging VOC.

Gering Eben、Azarian Taj、Schmedes Sarah、Duncan George、Colbert Jacob、Weiss James、Motes Jessy、Lopez Joe、Tekin Omer、Blanton Jason

Nova Southeastern UniversityBureau of Public Health Laboratories, Florida Department of HealthBureau of Public Health Laboratories, Florida Department of HealthNova Southeastern UniversityNova Southeastern UniversityBureau of Public Health Laboratories, Florida Department of HealthBureau of Public Health Laboratories, Florida Department of HealthNova Southeastern UniversityBureau of Public Health Laboratories, Florida Department of HealthBureau of Public Health Laboratories, Florida Department of Health

10.1101/2021.04.08.21255119

医学研究方法环境科学理论分子生物学

Gering Eben,Azarian Taj,Schmedes Sarah,Duncan George,Colbert Jacob,Weiss James,Motes Jessy,Lopez Joe,Tekin Omer,Blanton Jason.ddPCR Reveals SARS-CoV-2 Variants in Florida Wastewater[EB/OL].(2025-03-28)[2025-05-23].https://www.medrxiv.org/content/10.1101/2021.04.08.21255119.点此复制

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