Relocation of macrophages maintains the barrier function of the urothelium and protects against persistent infection
Relocation of macrophages maintains the barrier function of the urothelium and protects against persistent infection
SUMMARY Macrophages perform essential functions during bacterial infections, such as phagocytosis of pathogens and elimination of neutrophils to reduce spreading of infection, inflammation and tissue damage. The spatial distribution of macrophages is critical to respond to tissue specific adaptations upon infections. Using a novel algorithm for correlative mass spectrometry imaging and state-of-the-art multiplex microscopy, we report here that macrophages within the urinary bladder are positioned in the connective tissue underneath the urothelium. Invading uropathogenic E.coli induced an IL-6–dependent CX3CL1 expression by urothelial cells, facilitating relocation of macrophages from the connective tissue into the urothelium. These cells phagocytosed UPECs and eliminated neutrophils to maintain barrier function of the urothelium, preventing persistent and recurrent urinary tract infection. biorxiv;649137v1/UFIG1F1ufig1GRAPHICAL ABSTRACT
Uszkoreit Julian、Vijitha Nirojah、Soun Camille、Dixit Akanksha、Beerlage Anna-Lena、Eisenacher Martin、Engel Daniel R、Bracht Thilo、Urbanek Annett、Volke Julia K、Thiebes Stephanie、Horstmann Marius、Hoffmann Franziska、von Eggeling Ferdinand、Bottek Jenny、Sitek Barbara
Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-CenterInstitute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital EssenInstitute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital EssenInstitute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital EssenInstitute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital EssenRuhr University Bochum, Medical Faculty, Medizinisches Proteom-CenterInstitute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital EssenRuhr University Bochum, Medical Faculty, Medizinisches Proteom-CenterLeibniz Institute of Photonic TechnologyInstitute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital EssenInstitute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital EssenInstitute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital EssenDepartment of Otorhinolaryngology, Jena University HospitalDepartment of Otorhinolaryngology, Jena University HospitalInstitute of Experimental Immunology and Imaging, Department of Immunodynamics, University Hospital EssenRuhr University Bochum, Medical Faculty, Medizinisches Proteom-Center
基础医学生物科学研究方法、生物科学研究技术微生物学
Uszkoreit Julian,Vijitha Nirojah,Soun Camille,Dixit Akanksha,Beerlage Anna-Lena,Eisenacher Martin,Engel Daniel R,Bracht Thilo,Urbanek Annett,Volke Julia K,Thiebes Stephanie,Horstmann Marius,Hoffmann Franziska,von Eggeling Ferdinand,Bottek Jenny,Sitek Barbara.Relocation of macrophages maintains the barrier function of the urothelium and protects against persistent infection[EB/OL].(2025-03-28)[2025-05-12].https://www.biorxiv.org/content/10.1101/649137.点此复制
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