BBSome regulation of LITE-1 receptor in a cilium-independent manner in C. elegans
BBSome regulation of LITE-1 receptor in a cilium-independent manner in C. elegans
Abstract Bardet-Biedl Syndrome (BBS) is a genetic disorder affecting primary cilia. BBSome, a protein complex composed of eight BBS proteins, regulates the structure and function of cilia in diverse organisms, and its malfunction causes BBS in humans. Here, we report a new function of BBSome in C. elegans. In a forward genetic screen for genes regulating the light sensitivity of the ciliated ASH sensory neurons, we isolated bbs mutants, indicating that BBSome regulates ASH photosensitivity. Surprisingly, cilia are not required for ASH neurons to sense light, suggesting that BBSome regulates ASH photosensitivity independently of cilia. Interestingly, the light-sensing receptor LITE-1, which mediates photosensation, is a non-ciliary protein in ASH neurons. LITE-1 in ASH neurons becomes unstable in bbs mutants in an age-dependent manner, indicating that BBSome regulates the stability of LITE-1 in these neurons. These results identify a cilium-independent function of BBSome in regulating a non-ciliary protein in ciliated cells.
Liu Jianfeng、Zhang Xinxing、Xu X.Z. Shawn、Liu Jinzhi
College of Life Science and Technology, Key Laboratory of Molecular Biophysics of MOE, International Research Center for Sensory Biology and Technology of MOST, Huazhong University of Science and TechnologyLife Sciences Institute, University of Michigan||Department of Molecular and Integrative Physiology, University of Michigan Medical SchoolLife Sciences Institute, University of Michigan||Department of Molecular and Integrative Physiology, University of Michigan Medical SchoolLife Sciences Institute, University of Michigan||Department of Molecular and Integrative Physiology, University of Michigan Medical School||College of Life Science and Technology, Key Laboratory of Molecular Biophysics of MOE, International Research Center for Sensory Biology and Technology of MOST, Huazhong University of Science and Technology
遗传学细胞生物学分子生物学
Liu Jianfeng,Zhang Xinxing,Xu X.Z. Shawn,Liu Jinzhi.BBSome regulation of LITE-1 receptor in a cilium-independent manner in C. elegans[EB/OL].(2025-03-28)[2025-04-28].https://www.biorxiv.org/content/10.1101/2020.04.27.064998.点此复制
评论