MIC-MAC: High-throughput analysis of three-dimensional microglia morphology in mammalian brains
MIC-MAC: High-throughput analysis of three-dimensional microglia morphology in mammalian brains
ABSTRACT Microglia, the resident immune cells of the brain, exhibit complex and diverse phenotypes depending on their physiological context including brain regions and disease states. While single-cell RNA-sequencing has recently resolved this heterogeneity, it does not capture tissue and intercellular mechanisms. Our complementary imaging-based approach enables automatic 3D-morphology characterization and classification of thousands of individual microglia in situ and revealed species- and disease-specific morphological phenotypes in mouse and human brain samples.
Mechawar Naguib、Skupin Alexander、Salamanca Luis、Balling Rudi、Bouvier David S.、Murai Keith K.
Douglas Mental Health University Institute, Department of Psychiatry, McGill University, Montreal, QuebecLuxembourg Centre for Systems Biomedicine, University of Luxembourg, 7, Rue de Swing, Belval, L-4367||National Biomedical Computation Resource, University California San Diego, La Jolla, CALuxembourg Centre for Systems Biomedicine, University of Luxembourg, 7, Rue de Swing, Belval, L-4367Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 7, Rue de Swing, Belval, L-4367Luxembourg Centre for Systems Biomedicine, University of Luxembourg, 7, Rue de Swing, Belval, L-4367Centre for Research in Neuroscience, Department of Neurology and Neurosurgery, The Research Institute of the McGill University Health Centre, Montreal General Hospital, Montreal, Quebec
基础医学生物科学研究方法、生物科学研究技术神经病学、精神病学
Mechawar Naguib,Skupin Alexander,Salamanca Luis,Balling Rudi,Bouvier David S.,Murai Keith K..MIC-MAC: High-throughput analysis of three-dimensional microglia morphology in mammalian brains[EB/OL].(2025-03-28)[2025-05-07].https://www.biorxiv.org/content/10.1101/388397.点此复制
评论