Characterization of novel pollen-expressed transcripts reveals their potential roles in pollen heat stress response in Arabidopsis thaliana
Characterization of novel pollen-expressed transcripts reveals their potential roles in pollen heat stress response in Arabidopsis thaliana
ABSTRACT The male gametophyte is the most heat-sensitive of all plant tissues. In recent years, long noncoding RNAs (lncRNAs) have emerged as important components of cellular regulatory networks involved in most biological processes, including response to stress. While examining RNAseq datasets of developing and germinating Arabidopsis thaliana pollen exposed to HS, we identified 66 novel and 246 recently-annotated intergenic expressed loci (XLOCs) of unknown function, with the majority encoding lncRNAs. Comparison to HS in cauline leaves and other RNAseq experiments, indicated 74% of the 312 XLOCs are pollen-specific, and at least 42% are HS-responsive. Phylogenetic analysis revealed 96% of the genes evolved recently in Brassicaceae. We found that 50 genes are putative targets of microRNAs, and that 30% of the XLOCs contain small open reading frames (ORFs) with homology to protein sequences. Finally, RNAseq of ribosome-protected RNA fragments together with predictions of periodic footprint of the ribosome P-sites indicated that 23 of these ORFs are likely to be translated. Our findings indicate that many of the 312 unknown genes might be functional, and play significant role in pollen biology, including HSR.
Forment Javier、Luria Gilad、Schlauch Karen、Harper Jeffery、Miller Gad、Doniger Tirza、Tillet Richard、Poidevin Laetitia、Rath Abhishek、Ferrando Alejandro、Rutley Nicholas
Instituto de Biolog¨aa Molecular y Celular de Plantas CSIC/Universidad Polit¨|cnica de ValenciaThe Mina and Everard Goodman Faculty of Life Sciences Bar Ilan UniversityDepartment of Biochemistry and Molecular Biology, University of Nevada||Nevada INBRE Bioinformatics Core, University of Nevada RenoDepartment of Biochemistry and Molecular Biology, University of NevadaThe Mina and Everard Goodman Faculty of Life Sciences Bar Ilan UniversityThe Mina and Everard Goodman Faculty of Life Sciences Bar Ilan UniversityDepartment of Biochemistry and Molecular Biology, University of Nevada||Nevada INBRE Bioinformatics Core, University of Nevada RenoInstituto de Biolog¨aa Molecular y Celular de Plantas CSIC/Universidad Polit¨|cnica de ValenciaThe Mina and Everard Goodman Faculty of Life Sciences Bar Ilan UniversityInstituto de Biolog¨aa Molecular y Celular de Plantas CSIC/Universidad Polit¨|cnica de ValenciaThe Mina and Everard Goodman Faculty of Life Sciences Bar Ilan University
分子生物学植物学遗传学
Forment Javier,Luria Gilad,Schlauch Karen,Harper Jeffery,Miller Gad,Doniger Tirza,Tillet Richard,Poidevin Laetitia,Rath Abhishek,Ferrando Alejandro,Rutley Nicholas.Characterization of novel pollen-expressed transcripts reveals their potential roles in pollen heat stress response in Arabidopsis thaliana[EB/OL].(2025-03-28)[2025-05-11].https://www.biorxiv.org/content/10.1101/2020.08.20.258764.点此复制
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