Robustness of the ferret model for influenza risk assessment studies: a cross-laboratory exercise
Robustness of the ferret model for influenza risk assessment studies: a cross-laboratory exercise
Abstract Ferrets represent the preferred animal model for assessing the transmission potential of newly emerged zoonotic influenza viruses. However, heterogeneity among established experimental protocols and facilities across different laboratories may lead to variable results, complicating interpretation of transmission experimental data. Between 2018-2020, a global exercise was conducted by 11 participating laboratories to assess the range of variation in ferret transmission experiments using two common stock H1N1 influenza viruses that possess different transmission characteristics in ferrets. Inoculation route, dose, and volume were standardized, and all participating laboratories followed the same experimental conditions for respiratory droplet transmission, including a strict 1:1 donor:contact ratio. Additional host and environmental parameters likely to affect influenza transmission kinetics were monitored throughout. Overall transmission outcomes for both viruses across 11 laboratories were concordant, suggesting the robustness of the ferret model for zoonotic influenza risk assessment. To attain high confidence in identifying zoonotic influenza viruses with moderate-to-high or low transmissibility, our analyses support that as few as three but as many as five laboratories, respectively, would need to independently perform viral transmission experiments with concordant results. This exercise facilitates the development of a more homogenous protocol for ferret transmission experiments that are employed for the purposes of risk assessment.
Lau Eric HY、Chen Hualan、Fouchier Ron AM、Herfst Sander、Kawaoka Yoshihiro、Russell Charles、Sutton Troy C、Webby Richard J、Yang Huanliang、Yen Hui-Ling、Working group on the standardization of the ferret model for influenza risk assessment、Peiris Malik、Subbarao Kanta、Neumann Gabriele、Lakdawala Seema S、Perez Daniel R、Lee Leo Yi Yang、Belser Jessica A、Hatta Masato、Barr Ian G.、Barclay Wendy
School of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong KongState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural SciencesDepartment of Viroscience, Erasmus MCDepartment of Viroscience, Erasmus MCUniversity of WisconsinDepartment of Infectious Diseases, St. Jude Children?ˉs Research HospitalPennsylvania State UniversityDepartment of Infectious Diseases, St. Jude Children?ˉs Research HospitalState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural SciencesSchool of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong KongSchool of Public Health, Li Ka Shing Faculty of Medicine, The University of Hong KongWHO Collaborating Centre for Reference and Research on Influenza, VIDRL, Doherty Institute||Department of Microbiology and Immunology, University of Melbourne, Doherty InstituteUniversity of WisconsinUniversity of PittsburghUniversity of GeorgiaWHO Collaborating Centre for Reference and Research on Influenza, VIDRL, Doherty InstituteInfluenza Division, Centers for Disease Control and PreventionUniversity of WisconsinWHO Collaborating Centre for Reference and Research on Influenza, VIDRL, Doherty InstituteImperial College
医学研究方法基础医学微生物学
Lau Eric HY,Chen Hualan,Fouchier Ron AM,Herfst Sander,Kawaoka Yoshihiro,Russell Charles,Sutton Troy C,Webby Richard J,Yang Huanliang,Yen Hui-Ling,Working group on the standardization of the ferret model for influenza risk assessment,Peiris Malik,Subbarao Kanta,Neumann Gabriele,Lakdawala Seema S,Perez Daniel R,Lee Leo Yi Yang,Belser Jessica A,Hatta Masato,Barr Ian G.,Barclay Wendy.Robustness of the ferret model for influenza risk assessment studies: a cross-laboratory exercise[EB/OL].(2025-03-28)[2025-04-30].https://www.biorxiv.org/content/10.1101/2022.04.02.486825.点此复制
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