Comprehensive mapping of the human cytokine gene regulatory network
Comprehensive mapping of the human cytokine gene regulatory network
Summary Proper cytokine gene expression is essential in development, homeostasis, and immune responses. Studies on the transcriptional control of cytokine genes have mostly focused on highly researched transcription factors (TFs) and cytokines, resulting in an incomplete portrait of cytokine gene regulation. Here, we use enhanced yeast one-hybrid (eY1H) assays to derive a comprehensive network comprising 1,380 interactions between 265 TFs and 108 cytokine gene promoters, greatly expanding the known repertoire of TF-cytokine gene interactions. We found an enrichment of nuclear receptors and confirmed their role in cytokine regulation in primary macrophages. Additionally, we used the eY1H-derived network as a framework to identify pairs of TFs that synergistically modulate cytokine gene expression, and to identify novel TF-cytokine regulatory axes in immune diseases and immune cell lineage development. Overall, the eY1H data provides a rich resource to study cytokine regulation in a variety of physiological and disease contexts.
Yuan S、Gan KA、Henderson A、Carrasco Pro S、Fuxman Bass JI、Atianand MK、Lal S、Santoso CS、Sewell JA、Liu X、Agosto LM、Li Z
Department of Biology, Boston UniversityDepartment of Biology, Boston UniversityDepartment of Medicine, Section of Infectious Diseases, Boston University School of MedicineBioinformatics Program, Boston UniversityDepartment of Biology, Boston University||Bioinformatics Program, Boston UniversityDepartment of Immunology, University of PittsburghDepartment of Immunology, University of PittsburghDepartment of Biology, Boston UniversityDepartment of Biology, Boston UniversityDepartment of Biology, Boston UniversityDepartment of Medicine, Section of Infectious Diseases, Boston University School of MedicineBioinformatics Program, Boston University
基础医学生物科学研究方法、生物科学研究技术分子生物学
Yuan S,Gan KA,Henderson A,Carrasco Pro S,Fuxman Bass JI,Atianand MK,Lal S,Santoso CS,Sewell JA,Liu X,Agosto LM,Li Z.Comprehensive mapping of the human cytokine gene regulatory network[EB/OL].(2025-03-28)[2025-06-05].https://www.biorxiv.org/content/10.1101/2020.05.05.079657.点此复制
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