A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ
A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ
ABSTRACT Transmission electron microscopy (TEM) is a scientific research standard for producing nanometer-resolution ultrastructural images of subcellular components within cells and tissues. Mitochondria, endoplasmic reticulum (ER), lysosomes, and autophagosomes are organelles of particular interest to those investigating metabolic disorders. However, there is no clear consensus amongst regarding the best methods for quantifying the features of organelles in TEM images. In this protocol, we propose a standardized approach to accurately measure the morphology of these important subcellular structures using the free program ImageJ, developed by the National Institutes of Health (NIH). Specifically, we detail procedures for obtaining mitochondrial length, width, area, and circularity, in addition to assessing cristae morphology. We further provide methods for measuring interactions between the mitochondria and ER and measuring the length and width of lysosomes and autophagosomes. This standardized method can be used to quantify key features of organelle morphology, allowing investigators to produce accurate and reproducible measurements of organelle structures in their experimental samples. SUMMARYWe discuss a standardized method for measuring and quantifying organelle features using transmission electron microscopy and accessing for interactions between subcellular structures; organelles of focus include mitochondria, endoplasmic reticulum, lysosomes, and autophagosomes.
Mungai Margaret、Neikirk Kit、Abel E. Dale、Hinton Antentor Jr.、Katti Prasanna、AshShareef Salma、Lam Jacob、Pereira Renata O.、Salisbury Jeffrey L.、Lopez Edgar Garza、Glancy Brian、Christensen Trace A.、Biete Michelle
Department of Molecular and Cell Biology, University of California BerkeleyDaniel K. Inouye College of Pharmacy, University of Hawaii at HiloDepartment of Internal Medicine, University of Iowa ¨C Carver College of Medicine||Fraternal Order of Eagles Diabetes Research CenterDepartment of Molecular Physiology and Biophysics, Vanderbilt UniversityNational Heart, Lung, and Blood Institute, National Institutes of HealthDepartment of Internal Medicine, University of Iowa ¨C Carver College of Medicine||Fraternal Order of Eagles Diabetes Research CenterDepartment of Internal Medicine, University of Iowa ¨C Carver College of Medicine||Fraternal Order of Eagles Diabetes Research CenterDepartment of Internal Medicine, University of Iowa ¨C Carver College of Medicine||Fraternal Order of Eagles Diabetes Research CenterMicroscopy and Cell Analysis Core Facility, Mayo Clinic||Department of Biochemistry and Molecular Biology, Mayo ClinicDepartment of Molecular Physiology and Biophysics, Vanderbilt UniversityNational Heart, Lung, and Blood Institute, National Institutes of Health||National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of HealthMicroscopy and Cell Analysis Core Facility, Mayo ClinicDaniel K. Inouye College of Pharmacy, University of Hawaii at Hilo
医学研究方法基础医学生物科学研究方法、生物科学研究技术
Mungai Margaret,Neikirk Kit,Abel E. Dale,Hinton Antentor Jr.,Katti Prasanna,AshShareef Salma,Lam Jacob,Pereira Renata O.,Salisbury Jeffrey L.,Lopez Edgar Garza,Glancy Brian,Christensen Trace A.,Biete Michelle.A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ[EB/OL].(2025-03-28)[2025-04-29].https://www.biorxiv.org/content/10.1101/2021.05.27.446036.点此复制
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