线粒体基因与长跑运动员有氧耐力的关联性研究

Objective: The aerobic capacity is closely related to the molecular genetics of human mitochondrial DNA (mtDNA), and the frequencies distribution of seven loci of mtDNA were significant difference were found in previous studies (p<0.01) between long-distance athletes and controls. Therefore, this study aims to explore the relationship between mtDNA polymorphism and aerobic endurance index of long distance runners. Methods: 79 female long-distance runners, the movement of the main item for the first class athletes over the 5/10km or marathon, belonging to the northern Han population, were informed consent. Blood samples were collected from the subjects to extract whole blood DNA, and 7 polymorphic loci (mt235, mt514-515, mt16183, mt16290, mt16319, mt8794 and mt12338) of the polymorphic loci of the subjects were performed by micro sequencing. The oxygen endurance index, including anaerobic threshold heart rate (HRAT), anaerobic threshold running speed (VAT), anaerobic threshold intake oxygen (VO2AT), anaerobic threshold photo oxygen/weight (VO2AT/Kg) of maximum oxygen uptake (VO2), maximum perturbation oxygen/body weight (VO2max/Kg) and so on, were tested by increasing load treadmill protocol testing. Results: through correlation analysis and found no oxygen endurance index and mt235A/G, mt514-515CA/--, mt16183T/G, mt16290C/T, mt16319C/T, mt8794G/A and mt12338T/C significantly related subjects, indicating that the determinants of endurance index of the seven polymorphic loci is not long distance runners. Conclusion: This study did not find mtDNA polymorphism and long distance runners statistical differences in oxygen endurance index, shows that the movement is not the single polymorphic loci decision, may be the result of other factors.