National-scale surveillance of emerging SARS-CoV-2 variants in wastewater
National-scale surveillance of emerging SARS-CoV-2 variants in wastewater
Abstract SARS-CoV-2 surveillance is crucial to identify variants with altered epidemiological properties. Wastewater-based epidemiology (WBE) provides an unbiased and complementary approach to sequencing individual cases. Yet, national WBE surveillance programs have not been widely implemented and data analyses remain challenging. We deep-sequenced 2,093 wastewater samples representing 95 municipal catchments, covering >57% of Austria’s population, from December 2020 to September 2021. Our Variant Quantification in Sewage pipeline designed for Robustness (VaQuERo) enabled us to deduce variant abundance from complex wastewater samples and delineate the spatiotemporal dynamics of the dominant Alpha and Delta variants as well as regional clusters of other variants of concern. These results were cross validated by epidemiological records of >130,000 individual cases. Finally, we provide a framework to predict emerging variants de novo and infer variant-specific reproduction numbers from wastewater. This study demonstrates the power of national-scale WBE to support public health and promises particular value for countries without dense individual monitoring. Graphical Abstractmedrxiv;2022.01.14.21267633v1/UFIG1F1ufig1
Endler Lukas、Agerer Benedikt、Richter Lukas、Zechmeister Melanie、Heiler Georg、Penz Thomas、Senekowitsch Martin、Daleiden Beatrice、Niederst?tter Harald、Scheffknecht Christoph、Vogl Gunther、Popper Niki、Oberacher Herbert、Kreuzinger Norbert、Bergthaler Andreas、Radu Elena、Masseron Amandine、Bock Christoph、Triska Petr、Markt Rudolf、Insam Heribert、Thornton Matthew、Allerberger Franz、Weichlinger G¨1nther、Schmid Daniela、Bicher Martin、Hupfauf Sebastian、Amman Fabian、Wagner Andreas、Steinlechner Martin、Schedl Anna、Laine Jan、Slipko Katarzyna、Keszei Zsofia
CeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesAustrian Agency for Health and Food Safety (AGES)dwh GmbHComplexity Science Hub||Institute of Information Systems EngineeringCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesInstitute of Legal Medicine and Core Facility Metabolomics, Medical University of InnsbruckInstitute of Legal Medicine and Core Facility Metabolomics, Medical University of InnsbruckInstitut f¨1r Umwelt und Lebensmittelsicherheit des Landes VorarlbergInstitut f¨1r Lebensmittelsicherheit, Veterin?rmedizin und Umwelt des Landes K?rntendwh GmbH||Institute for Information Systems EngineeringInstitute of Legal Medicine and Core Facility Metabolomics, Medical University of InnsbruckInstitute for Water Quality and Resource ManagementCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesInstitute for Water Quality and Resource Management||?tefan S. Nicolau Institute of VirologyInstitute for Water Quality and Resource ManagementCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesDepartment of Microbiology, University of InnsbruckDepartment of Microbiology, University of InnsbruckCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesAustrian Agency for Health and Food Safety (AGES)Abteilung 12 ¨C Wasserwirtschaft, Amt der K?rntner LandesregierungAustrian Agency for Health and Food Safety (AGES)dwh GmbH||Institute for Information Systems EngineeringDepartment of Microbiology, University of InnsbruckCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesDepartment of Microbiology, University of InnsbruckInstitute of Legal Medicine and Core Facility Metabolomics, Medical University of InnsbruckCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesCeMM Research Center for Molecular Medicine of the Austrian Academy of SciencesInstitute for Water Quality and Resource ManagementCeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences
医学研究方法预防医学环境科学理论
Endler Lukas,Agerer Benedikt,Richter Lukas,Zechmeister Melanie,Heiler Georg,Penz Thomas,Senekowitsch Martin,Daleiden Beatrice,Niederst?tter Harald,Scheffknecht Christoph,Vogl Gunther,Popper Niki,Oberacher Herbert,Kreuzinger Norbert,Bergthaler Andreas,Radu Elena,Masseron Amandine,Bock Christoph,Triska Petr,Markt Rudolf,Insam Heribert,Thornton Matthew,Allerberger Franz,Weichlinger G¨1nther,Schmid Daniela,Bicher Martin,Hupfauf Sebastian,Amman Fabian,Wagner Andreas,Steinlechner Martin,Schedl Anna,Laine Jan,Slipko Katarzyna,Keszei Zsofia.National-scale surveillance of emerging SARS-CoV-2 variants in wastewater[EB/OL].(2025-03-28)[2025-07-01].https://www.medrxiv.org/content/10.1101/2022.01.14.21267633.点此复制
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