Metagenomic sequencing of municipal wastewater provides a near-complete SARS-CoV-2 genome sequence identified as the B.1.1.7 variant of concern from a Canadian municipality concurrent with an outbreak
Metagenomic sequencing of municipal wastewater provides a near-complete SARS-CoV-2 genome sequence identified as the B.1.1.7 variant of concern from a Canadian municipality concurrent with an outbreak
Abstract Laboratory-based wastewater surveillance for SARS-CoV-2, the causative agent of the ongoing COVID-19 pandemic, can be conducted using RT-qPCR-based screening of municipal wastewater samples. Although it provides rapid viral detection and can inform SARS-CoV-2 abundance in wastewater, this approach lacks the resolution required for viral genotyping and does not support tracking of viral genome evolution. The recent emergence of several variants of concern, a result of mutations across the genome including the accrual of important mutations within the viral spike glycoprotein, has highlighted the need for a method capable of detecting the cohort of mutations associated with these and newly emerging genotypes. Here we provide an innovative methodology for the recovery of a near-complete SARS-CoV-2 sequence from a wastewater sample collected from across Canadian municipalities including one that experienced a significant outbreak attributable to the SARS-CoV-2 B.1.1.7 variant of concern. Our results demonstrate that a combined interrogation of genome consensus-level sequences and alternative alleles enables the identification of a SARS-CoV-2 variant of concern and the detection of a new allele within a viral accessory gene that may be representative of a recently evolved B.1.1.7 sublineage.
Ali Jennifer、Robert Erin、Nadon Celine、Buchanan Cody、Schonfeld Justin、Wells Matthew、Landgraff Chrystal、Wang Lu Ya Ruth、Bessonov Kyrylo
Division of Enteric Diseases, National Microbiology LaboratoryDivision of Enteric Diseases, National Microbiology LaboratoryDivision of Enteric Diseases, National Microbiology Laboratory||Department of Medical Microbiology and Infectious Diseases, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of ManitobaDivision of Enteric Diseases, National Microbiology LaboratoryDivision of Enteric Diseases, National Microbiology Laboratory, Public Health Agency of CanadaDivision of Enteric Diseases, National Microbiology LaboratoryDivision of Enteric Diseases, National Microbiology Laboratory||Food Science Department, University of Guelph, GuelphDivision of Enteric Diseases, National Microbiology LaboratoryDivision of Enteric Diseases, National Microbiology Laboratory, Public Health Agency of Canada
医学研究方法环境科学理论微生物学
Ali Jennifer,Robert Erin,Nadon Celine,Buchanan Cody,Schonfeld Justin,Wells Matthew,Landgraff Chrystal,Wang Lu Ya Ruth,Bessonov Kyrylo.Metagenomic sequencing of municipal wastewater provides a near-complete SARS-CoV-2 genome sequence identified as the B.1.1.7 variant of concern from a Canadian municipality concurrent with an outbreak[EB/OL].(2025-03-28)[2025-08-09].https://www.medrxiv.org/content/10.1101/2021.03.11.21253409.点此复制
评论