Combined single-cell and spatial transcriptomics reveals the molecular, cellular and spatial bone marrow niche organization
Combined single-cell and spatial transcriptomics reveals the molecular, cellular and spatial bone marrow niche organization
SUMMARY The bone marrow (BM) constitutes the primary site for life-long blood production and skeletal regeneration. However, its cellular composition and the spatial organization into distinct ‘niches’ remains controversial. Here, we combine single-cell and spatially resolved transcriptomics to systematically map the molecular and cellular composition of the endosteal, sinusoidal, and arteriolar BM niches. This allowed us to transcriptionally profile all major BM resident cell types, determine their localization, and clarify the cellular and spatial sources of key growth factors and cytokines. Our data demonstrate that previously unrecognized Cxcl12-abundant reticular (CAR) cell subsets (i.e. Adipo- and Osteo-CAR cells) differentially localize to sinusoidal or arteriolar surfaces, locally act as ‘professional cytokine secreting cells’, and thereby establish distinct peri-vascular micro-niches. Importantly, we also demonstrate that the 3-dimensional organization of the BM can be accurately inferred from single-cell gene expression data using the newly developed RNA-Magnet algorithm. Together, our study reveals the cellular and spatial organization of BM niches, and offers a novel strategy to dissect the complex organization of whole organs in a systematic manner. One Sentence SummaryIntegration of single-cell and spatial transcriptomics reveals the molecular, cellular and spatial organization of bone marrow niches
Baccin Chiara、Hern¨￠ndez-Malmierca Pablo、Nombela-Arrieta C¨|sar、Trumpp Andreas、Gr¨1nschl?ger Florian、Velten Lars、Steinmetz Lars M.、Al-Sabah Jude、Helbling Patrick M.、Haas Simon
European Molecular Biology Laboratory (EMBL)||Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of BiosciencesHeidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)||Division of Stem Cells and Cancer, Deutsches Krebsforschungszentrum (DKFZ) and DKFZ-ZMBH AllianceDepartment of Medical Oncology and Hematology, University Hospital and University of ZurichHeidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)||Division of Stem Cells and Cancer, Deutsches Krebsforschungszentrum (DKFZ) and DKFZ-ZMBH Alliance||German Cancer Consortium (DKTK)Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)||Division of Stem Cells and Cancer, Deutsches Krebsforschungszentrum (DKFZ) and DKFZ-ZMBH AllianceEuropean Molecular Biology Laboratory (EMBL)European Molecular Biology Laboratory (EMBL)||Department of Genetics, Stanford University School of Medicine||Stanford Genome Technology CenterHeidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)||Division of Stem Cells and Cancer, Deutsches Krebsforschungszentrum (DKFZ) and DKFZ-ZMBH AllianceDepartment of Medical Oncology and Hematology, University Hospital and University of ZurichHeidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH)||Division of Stem Cells and Cancer, Deutsches Krebsforschungszentrum (DKFZ) and DKFZ-ZMBH Alliance
基础医学细胞生物学分子生物学
Baccin Chiara,Hern¨￠ndez-Malmierca Pablo,Nombela-Arrieta C¨|sar,Trumpp Andreas,Gr¨1nschl?ger Florian,Velten Lars,Steinmetz Lars M.,Al-Sabah Jude,Helbling Patrick M.,Haas Simon.Combined single-cell and spatial transcriptomics reveals the molecular, cellular and spatial bone marrow niche organization[EB/OL].(2025-03-28)[2025-07-18].https://www.biorxiv.org/content/10.1101/718395.点此复制
评论